ReviewMolecular biotechnology2019
Design of Experiments As a Tool for Optimization in Recombinant Protein Biotechnology: From Constructs to Crystals.
Review in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Strategy for Improving the Diagnostic Efficacy of Bovine Tuberculosis Using a Novel PCRVeterinary research communications · 2026Article
- Transfer learning Bayesian optimization for competitor DNA molecule design for use in diagnostic assays.Biotechnology and bioengineering · 2025Article
- Optimizing recombinant mini proinsulin production via response surface method and microbioreactor screening.PloS one · 2025Article
- Transfer Learning Bayesian Optimization to Design Competitor DNA Molecules for Use in Diagnostic Assays.ArXiv · 2024Article
- Article
- Indirect optimization of staphylokinase expression level in dicistronic auto-inducible system.AMB Express · 2022Article
- Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01.Scientific reports · 2022Article
- Quality Management Tools on the Stage: Old but New Allies for Rigor and Standardization of Extracellular Vesicle Studies.Frontiers in bioengineering and biotechnology · 2022Article
- Review
- Design of transfections: Implementation of design of experiments for cell transfection fine tuning.Biotechnology and bioengineering · 2021Article
- Optimization of Expression and Purification of Schistosoma mansoni Antigens in Fusion with Rhizavidin.Molecular biotechnology · 2021Article
- Heterologous and Homologous Expression of Proteins from Haloarchaea: Denitrification as Case of Study.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this review, the basic concepts and applications of design of experiments (DoE) in recombinant protein biotechnology will be discussed. The production of recombinant proteins usually begins with the construction of an expression vector that is then introduced into a microbial host. The target protein is overexpressed in the host's cells and subsequently, it is isolated using a suitable purification method, its activity is assessed using a biological assay, while its crystallization is often required. Because each protein is unique and due to the complex interactions among the reagents in experiments, it is impossible that one set of reaction conditions would be optimal for all cases. Optimization of experimental conditions is usually carried out by the inefficient one-factor-at-a-time approach that does not take into account the combined effects of factors on a process. On the other hand, DoE approaches with a carefully selected small set of experiments, and therefore with a reduced cost and in a limited amount of time predict the effect of each factor and the effects of their interactions on a process. Importantly, several software packages are available that facilitate the choice of the DoE approach, design of the experiments, and analysis of the results.
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Registered trials
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