ArticleCurrent microbiology2020
Isolation and Genome Sequencing of a Novel Pseudomonas aeruginosa Phage PA-YS35.
Article in Current microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- A comprehensive analysis of the kinetics of infection of lytic bacteriophages specific to the ESKAPE and critical pathogens.World journal of microbiology & biotechnology · 2026Review
- Isolation and characterization of a lytic phage and its efficacy against multi-drug resistant strains of pseudomonas aeruginosa.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Isolation and Characterization of Three Lytic Bacteriophages to Overcome Multidrug-, Extensive Drug-, and Pandrug-ResistantPHAGE (New Rochelle, N.Y.) · 2024Article
- A single-layer spot assay for easy, fast, and high-throughput quantitation of phages against multidrug-resistant Gram-negative pathogens.Journal of clinical microbiology · 2024Article
- Genome sequencing ofMicrobiology resource announcements · 2024Article
- Complete Genome Sequencing of a Novel Pseudomonas aeruginosa Phage, UF_RH5.Microbiology resource announcements · 2023Article
- Complete Genome Sequence of Pseudomonas aeruginosa Phage UF_RH6, Isolated from Human Lung.Microbiology resource announcements · 2023Article
- Complete Genome Sequencing of the Novel Pseudomonas aeruginosa Phage UF_RH1.Microbiology resource announcements · 2023Article
- Resistance ofMicrobiology spectrum · 2023Article
- Characterization of Phage Resistance and Their Impacts on Bacterial Fitness in Pseudomonas aeruginosa.Microbiology spectrum · 2022Article
- Isolation and Characterization of LyticViruses · 2022Article
- T4-like Bacteriophages Isolated from Pig Stools InfectViruses · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Phage PA-YS35 is a novel lytic Pseudomonas aeruginosa phage belonging to the Myoviridae family and was isolated from the sewage of the First Hospital of Jilin University. The biological properties testing indicated that phage PA-YS35 is stable between - 20 and 60 °C and pH 4-9. The one-step growth curve shows that the latent period of PA-YS35 was 9 min, and the burst period was about 21 min by the size of approximately 380 progeny phages per host cell. The genome of phage PA-YS35 is linear double-stranded DNA with a size of 93,296 bp and a GC content of 49.35%. The results from RAST gene annotation analysis showed that the PA-YS35 genome contains 172 open reading frames (ORFs); the function of 41 ORFs can be predicted, whereas the product of remaining 131 ORFs are hypothetical proteins. According to phylogenetic tree of RNA ligase encoding sequence, phage PA-YS35 has a close evolutionary relationship with Pseudomonas phage PAK P1 because both of them are located on the same branch. The study of phage PA-YS35 genome will provide useful information for further research on the interaction between phages and their hosts.
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Registered trials
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