Evidence map›Paper›PMID 31664073›Full record

ArticleScientific reports2019

LXRβ controls glioblastoma cell growth, lipid balance, and immune modulation independently of ABCA1.

Deven Patel, Fahim Ahmad, Diane M Kambach, Qian Sun, Alan S Halim, Tamalee Kramp, Kevin A Camphausen, Jayne M Stommel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
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  4. NOVA1 acts as an oncogenic RNA-binding protein to regulate cholesterol homeostasis in human glioblastoma cells.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Metabolic reprogramming in prostate cancer.British journal of cancer · 2021
    Review
  14. Review
  15. Article
  16. Metabolic Drivers of Invasion in Glioblastoma.Frontiers in cell and developmental biology · 2021
    Review
  17. Old Stars and New Players in the Brain Tumor Microenvironment.Frontiers in cellular neuroscience · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Deven PatelRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Fahim AhmadRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID http://orcid.org/0000-0002-7216-5539
Diane M KambachRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Qian SunRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Alan S HalimRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Tamalee KrampRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Kevin A CamphausenRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Jayne M StommelRadiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA. jayne.stommel@nih.gov.ORCID http://orcid.org/0000-0003-2485-7992
National Institutes of Health · US

Funding

Cell density effects on therapeutic sensitivityZIABC011441 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI STOMMEL, JAYNE · 2012 to 2019
$4.0M
Intramural NIH HHS ZIA BC011441
6 · The paper itself

Abstract

Cholesterol is a critical component of membranes and a precursor for hormones and other signaling molecules. Previously, we showed that unlike astrocytes, glioblastoma cells do not downregulate cholesterol synthesis when plated at high density. In this report, we show that high cell density induces ABCA1 expression in glioblastoma cells, enabling them to get rid of excess cholesterol generated by an activated cholesterol biosynthesis pathway. Because oxysterols are agonists for Liver X Receptors (LXRs), we investigated whether increased cholesterol activates LXRs to maintain cholesterol homeostasis in highly-dense glioblastoma cells. We observed that dense cells had increased oxysterols, which activated LXRβ to upregulate ABCA1. Cells with CRISPR-mediated knockdown of LXRβ, but not ABCA1, had decreased cell cycle progression and cell survival, and decreased feedback repression of the mevalonate pathway in densely-plated glioma cells. LXRβ gene expression poorly correlates with ABCA1 in glioblastoma patients, and expression of each gene correlates with poor patient prognosis in different prognostic subtypes. Finally, gene expression and lipidomics analyses cells revealed that LXRβ regulates the expression of immune response gene sets and lipids known to be involved in immune modulation. Thus, therapeutic targeting of LXRβ in glioblastoma might be effective through diverse mechanisms.

Indexed as

Lipid MetabolismATP Binding Cassette Transporter 1Brain NeoplasmsCell ProliferationCholesterolGlioblastomaHomeostasisHumansLiver X ReceptorsMevalonic AcidSignal TransductionTranscription, GeneticABCA1 protein, humanATP Binding Cassette Transporter 1CholesterolLiver X ReceptorsMevalonic Acid

Identifiers

PMID31664073
PMCPMC6820787
OpenAlexW2982534011

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.