ArticleeLife2019
One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Murine Models of Obesity-Related Cancer Risk.Cancer prevention research (Philadelphia, Pa.) · 2025Review
- Pu.1/Spi1 dosage controls the turnover and maintenance of microglia in zebrafish and mammals.eLife · 2025Article
- A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish.PLoS genetics · 2025Article
- Base editors in zebrafish: a new era for functional genomics and disease modeling.Frontiers in genome editing · 2025Review
- CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit.Nature communications · 2024Article
- Control of cardiac contractions using Cre-lox and degron strategies in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A robust knock-in approach using a minimal promoter and a minicircle.Developmental biology · 2024Article
- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optimal tagging strategies for illuminating expression profiles of genes with different abundance in zebrafish.Communications biology · 2023Article
- The role of cilia during organogenesis in zebrafish.Open biology · 2023Review
- Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish.Developmental cell · 2023Article
- Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration.Protein & cell · 2023Article
- Efficient knock-in method enabling lineage tracing in zebrafish.Life science alliance · 2023Article
- A robust pipeline for efficient knock-in of point mutations and epitope tags in zebrafish using fluorescent PCR based screening.BMC genomics · 2022Article
- Promoting validation and cross-phylogenetic integration in model organism research.Disease models & mechanisms · 2022Article
- Cre/eLife · 2022Article
- Regenerative neurogenesis: the integration of developmental, physiological and immune signals.Development (Cambridge, England) · 2022Article
- Article
- zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Zebrafish as a Model to Study Vascular Elastic Fibers and Associated Pathologies.International journal of molecular sciences · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no 'two-in-one' KI strategy combining conditional knockout (CKO) with fluorescent gene-labeling or further allele-labeling has been reported. Here, we developed a dual-cassette-donor strategy and achieved one-step and efficient generation of dual-function KI alleles at
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.