ArticleNon-coding RNA2019
Accurate Adapter Information Is Crucial for Reproducibility and Reusability in Small RNA Seq Studies.
Article in Non-coding RNA, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Advances in methods for tRNA sequencing and quantification.Trends in genetics : TIG · 2024Review
- FindAdapt: A python package for fast and accurate adapter detection in small RNA sequencing.PLoS computational biology · 2024Article
- Adapter dimer contamination in sRNA-sequencing datasets predicts sequencing failure and batch effects and hampers extracellular vesicle-sRNA analysis.Journal of extracellular biology · 2023Article
- Globally Accessible Distributed Data Sharing (GADDS): a decentralized FAIR platform to facilitate data sharing in the life sciences.Bioinformatics (Oxford, England) · 2022Article
- dbDEMC 3.0: Functional Exploration of Differentially Expressed miRNAs in Cancers of Human and Model Organisms.Genomics, proteomics & bioinformatics · 2022Article
- A bioinformatics approach to microRNA-sequencing analysis.Osteoarthritis and cartilage open · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A necessary pre-processing data analysis step is the removal of adapter sequences from the raw reads. While most adapter trimming tools require adapter sequence as an essential input, adapter information is often incomplete or missing. This can impact quantification of features, reproducibility of the study and might even lead to erroneous conclusions. Here, we provide examples to highlight the importance of specifying the adapter sequence by demonstrating the effect of using similar but different adapter sequences and identify additional potential sources of errors in the adapter trimming step. Finally, we propose solutions by which users can ensure their small RNA-seq data is fully annotated with adapter information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.