ArticleJournal of biophotonics2020
Lightsheet fluorescence lifetime imaging microscopy with wide-field time-correlated single photon counting.
Article in Journal of biophotonics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Luminescence lifetime imaging integrated into selective plane illumination microscopy for oxygen imaging in 3D cell cultures.Biomedical optics express · 2026Article
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.Communications biology · 2024Article
- Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D.Current opinion in solid state & materials science · 2024Article
- A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos.Scientific reports · 2024Article
- Multiplexed near infrared fluorescence lifetime imaging in turbid media.Journal of biomedical optics · 2024Article
- Visualization of Nanocarriers and Drugs in Cells and Tissue.Handbook of experimental pharmacology · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- Förster resonance energy transfer efficiency measurements on vinculin tension sensors at focal adhesions using a simple and cost-effective setup.Journal of biomedical optics · 2023Article
- Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.Journal of biomedical optics · 2023Article
- Light sheet autofluorescence lifetime imaging with a single photon avalanche diode array.bioRxiv : the preprint server for biology · 2023Article
- Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine.Journal of biomedical optics · 2021Article
- A Dual Fluorescence-Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM-EPR Spectroscopy.Angewandte Chemie (International ed. in English) · 2021Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.Current protocols in neuroscience · 2020Review
- Lightsheet fluorescence lifetime imaging microscopy with wide-field time-correlated single photon counting.Journal of biophotonics · 2020Article
- Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue.Theranostics · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
We report on wide-field time-correlated single photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) with lightsheet illumination. A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single-photon sensitive camera, is used to record the position and arrival time of the photons with picosecond time resolution, combining low illumination intensity of microwatts with wide-field data collection. We pair this detector with the lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients in human cancer cell spheroids, and C. elegans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.