ArticlePloS one2019
Detection and genetic characterization of Echinococcus granulosus mitochondrial DNA in serum and formalin-fixed paraffin embedded cyst tissue samples of cystic echinococcosis patients.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- High efficacy of an in-house antigen B ELISA for diagnosing human cystic echinococcosis compared with a commercial kit and a cell-free DNA assay.Scientific reports · 2026Article
- A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.Cell reports methods · 2026Article
- Molecular evidence of Echinococcus canadensis (G6/G7) predominance in Mongolian livestock and its implications for control.PLoS neglected tropical diseases · 2026Article
- Enhancing hydatid cysts diagnosis utilizing cell-free DNA as a sensitive biomarker forHelminthologia · 2026Article
- Three decades of research on molecular genetic characteristics of Echinococcus spp.: a bibliometric study.Folia parasitologica · 2026Review
- Advances in single-cell transcriptomic sequencing in hepatic echinococcosis.Frontiers in immunology · 2026Review
- Frequency and Genetic Characterization ofJournal of parasitology research · 2026Article
- The Role of Innate Lymphoid Cells in the Pathogenesis of Hepatic Echinococcosis: A Literature Review.Parasite immunology · 2026Review
- Molecular diagnosis of suspected intestinal schistosomiasis in a non-endemic area of Yunnan Province, China.Infectious diseases of poverty · 2025Article
- A ten-year evaluation of central nervous system cystic echinococcosis in a highly endemic area of Iran: Molecular epidemiology and clinicopathological characteristics.Parasite epidemiology and control · 2025Article
- Detection of circulatory E. granulosus-derived cell-free DNA in the plasma and urine of human cystic echinococcosis using an in-house PCR: a potential promising diagnostic biomarker.Molecular biology reports · 2024Article
- Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis.PLoS neglected tropical diseases · 2023Article
- Genetic characterization of human echinococcosis in Southern Punjab, Pakistan.Frontiers in cellular and infection microbiology · 2023Article
- Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran.Journal of cardiothoracic surgery · 2021Article
- Establishing and evaluation of a polymerase chain reaction for the detection of Echinococcus multilocularis in human tissue.PLoS neglected tropical diseases · 2021Article
- Epidemiological, Morphometric, and Molecular Investigation of Cystic Echinococcosis in Camel and Cattle From Upper Egypt: Current Status and Zoonotic Implications.Frontiers in veterinary science · 2021Article
- Diagnostic urinary cfDNA detected in human cystic echinococcosis.Molecular and biochemical parasitology · 2020Article
- Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing.PLoS neglected tropical diseases · 2020Article
- Histopathologic Alterations betweenIranian journal of parasitologyArticle
- Molecular diagnosis of echinococcosis in patients based on frozen paraffin tissue samples and fixed formalin and hydatid cysts isolated from livestock in a slaughterhouse.Tropical parasitologyArticle
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5 authors at 1 institution in 1 country.
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Abstract
Cystic echinococcosis (CE) is a worldwide zoonotic disease caused by the larval stage of Echinococcus granulosus. We investigated the presence of E. granulosus-specific DNA in the serum of CE patients by detecting the cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit I (nad1) mitochondrial genes. Serum and formalin-fixed paraffin embedded (FFPE) cyst tissue samples of 80 CE patients were analyzed. The extracted DNA of samples was submitted to PCR amplification of cox1 and nad1 genes, and products were sequenced and genotyped. Nineteen (23.8%; 95% CI 15.8-34.1) serum and 78 (97.5%; 95% CI 91.3-99.3) FFPE cyst tissue samples were successfully amplified with at least one gene. Echinococcus DNA was detected in the sera of 15.0% (95% CI: 8.8-24.4) and 10.0% (95% CI: 5.2-18.5) and in cyst tissue of 91.3% (95% CI: 83.0-95.7) and 83.8% (95% CI: 74.2-90.3) of 80 patients by cox1 and nad1 gene, respectively. Four genotypes of E. granulosus were distinguished in the CE patients, with predominance of genotype G1, followed by G3, G2, and G6. The finding of E. granulosus DNA in 23.8% of serum samples from CE patients confirmed that E. granulosus releases cell-free DNA into the circulatory system, but quantities may be inadequate for the diagnosis of CE. Genotype G1 predominance suggests the sheep-dog cycle as the primary route of human infection.
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