Evidence map›Paper›PMID 31661016›Full record

ArticleGenome biology2019

RaGOO: fast and accurate reference-guided scaffolding of draft genomes.

Michael Alonge, Sebastian Soyk, Srividya Ramakrishnan, Xingang Wang, Sara Goodwin, Fritz J Sedlazeck, Zachary B Lippman, Michael C Schatz

Abstract readEvaluation Study
In one paragraph

Article in Genome biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 422 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
422citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

422 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  4. Genome sequence of endophyteMicrobiology resource announcements · 2026
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  5. Chromosome-scale genome assembly and genomic prediction of essential oil compounds in Atractylodes lancea for genomics-assisted breeding.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026
    Article
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362 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Michael AlongeDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, USA.
Sebastian SoykCold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Srividya RamakrishnanDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, USA.
Xingang WangCold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Sara GoodwinCold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Fritz J SedlazeckHuman Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA.
Zachary B LippmanCold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Michael C SchatzDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, USA. mschatz@cs.jhu.edu.ORCID 0000-0002-4118-4446

Funding

Genomic Architecture of Common Disease in Diverse Populations: WGS of Ongoing Hemorrhagic Stroke Study SupplementUM1HG008898 · NHGRI · BAYLOR COLLEGE OF MEDICINE · PI GIBBS, RICHARD A · 2016 to 2020
$77.0M
Next Generation DNA SequencerS10OD020122 · OD · COLD SPRING HARBOR LABORATORY · PI MCCOMBIE, WILLIAM RICHARD · 2015 to 2015
$523k
NIH HHS R01-HG006677NIH HHS S10 OD020122NIH HHS UM1 HG008898
6 · The paper itself

Abstract

We present RaGOO, a reference-guided contig ordering and orienting tool that leverages the speed and sensitivity of Minimap2 to accurately achieve chromosome-scale assemblies in minutes. After the pseudomolecules are constructed, RaGOO identifies structural variants, including those spanning sequencing gaps. We show that RaGOO accurately orders and orients 3 de novo tomato genome assemblies, including the widely used M82 reference cultivar. We then demonstrate the scalability and utility of RaGOO with a pan-genome analysis of 103 Arabidopsis thaliana accessions by examining the structural variants detected in the newly assembled pseudomolecules. RaGOO is available open source at https://github.com/malonge/RaGOO .

Indexed as

SoftwareArabidopsisGenome, PlantGenomicsGenomic Structural VariationSolanum lycopersicumGenome alignmentGenome assemblyLong-read sequencingPseudomoleculeReference-guidedScaffoldingTomato

Identifiers

PMID31661016
PMCPMC6816165

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.