ArticleBMC infectious diseases2019
Chromosomal and plasmid-mediated fluoroquinolone resistance in human Salmonella enterica infection in Ghana.
Article in BMC infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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23 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- A systematic review and meta-analysis on antibiotic resistance genes in Ghana.BMC medical genomics · 2025Pooled it
- Gene Expression Analysis and Whole Genome Sequencing Reveal the Potential Mechanism of Ciprofloxacin Resistance in aVeterinary sciences · 2026Article
- Genomic characterization of respiratoryFrontiers in microbiology · 2026Article
- Antibiotic susceptibility patterns of clinical isolates of salmonella species producing extended spectrum beta lactamases as predictor of multidrug resistance in a tertiary hospital, Southeastern Nigeria.BMC infectious diseases · 2025Article
- The molecular mechanisms of quinolone resistance in Salmonella: a review.Archives of microbiology · 2025Review
- Characterization and Antimicrobial Resistance of Non-TyphoidalMicroorganisms · 2025Article
- Prevalence and antimicrobial resistance patterns of nontyphoidal Salmonella in Ghana: a systematic review and meta-analysis.Tropical medicine and health · 2025Review
- A Comprehensive Review of Molecular Mechanisms Leading to the Emergence of Multidrug Resistance in Bacteria.Indian journal of microbiology · 2025Review
- Article
- Salmonella Typhi genotypic diversity, cluster identification and antimicrobial resistance determinants in Mukuru settlement, Nairobi Kenya.BMC infectious diseases · 2024Article
- Typhoidal salmonella disease in Mukuru informal settlement, Nairobi Kenya; carriage, diversity, and antimicrobial resistant genes.PloS one · 2024Article
- An Overview of Antimicrobial Resistance Profiles of Publicly AvailableFoodborne pathogens and disease · 2023Article
- Antimicrobial Resistance: Two-Component Regulatory Systems and Multidrug Efflux Pumps.Antibiotics (Basel, Switzerland) · 2023Review
- The Mechanisms Involved in the Fluoroquinolone Resistance ofPathogens (Basel, Switzerland) · 2023Article
- Co-existence of extended-spectrum β-lactamases blaEuropean journal of medical research · 2023Article
- Genomic alterations involved in fluoroquinolone resistance development in Staphylococcus aureus.PloS one · 2023Article
- Utilising cumulative antibiogram data to enhance antibiotic stewardship capacity in the Cape Coast Teaching Hospital, Ghana.Antimicrobial resistance and infection control · 2022Article
- Epidemiology of Plasmids inAntibiotics (Basel, Switzerland) · 2022Article
- Plasmid-mediated quinolone resistance determinants in clinical bacterial pathogens isolated from the Western Region of Ghana: a cross-sectional study.The Pan African medical journal · 2022Article
- Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in NontyphoidalAntibiotics (Basel, Switzerland) · 2021Article
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Authors and funding
13 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonella infection poses significant public health threat globally, especially in resource-limited countries. Emergence and spread of antibiotic resistant strains to fluoroquinolones have led to treatment failures and increased mortality in Salmonella infection. However, there is dearth of information regarding mechanisms of resistance to fluoroquinolones in Ghana. This study therefore sought to identify chromosomal mutations and plasmid-mediated resistance as possible mechanisms of fluoroquinolone resistance from clinical isolates in Ghana.
methodsThis was a retrospective study of archived isolates biobanked at Kumasi Centre for Collaborative Research in Tropical Medicine, Ghana. Isolates were obtained from blood, stool and oropharynx samples at two hospitals, between May, 2016 and January, 2018. Salmonella identification was done using standard microbiological protocols and antibiotic susceptibility testing performed by Kirby-Bauer disc diffusion method. Isolates with intermediate susceptibility and/or resistance to nalidixic acid and/or ciprofloxacin were selected and examined for chromosomal mutations by Sanger sequencing and plasmid-mediated resistance by PCR.
resultsOf 133 biobanked isolates cultured, 68 (51.1%) and 16 (12%) were identified as Salmonella Typhi and non-typhoidal Salmonella (NTS), respectively. Sequence analysis of gyrA gene revealed the presence of 5 different nonsynonymous mutations, with the most frequent mutation (Ile203Ser) occurring in 12 out of 13 isolates tested. Gyrase B (gyrB) gene had 1 nonsynonymous mutation in 3 out of 13 isolates, substituting phenylalanine with leucine at codon 601 (Phe601Leu). No mutation was observed in parC and parE genes. Two NTS isolates were found to harbour qnrS plasmid-mediated resistant gene of molecular size 550 bp with high ciprofloxacin MIC of 0.5 μg/ml.
conclusionThis study reports for the first time in Ghana plasmid-mediated fluoroquinolone resistant gene qnrS in Salmonella clinical isolates. Nonsynonymous mutations of gyrA and gyrB genes likely to confer Salmonella reduced susceptibility to ciprofloxacin were also reported.
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