ArticleAnalytical chemistry2019
Quantitative Imaging of Proteins in Tissue by Stable Isotope Labeled Mimetic Liquid Extraction Surface Analysis Mass Spectrometry.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Quantitative Mass Spectrometry Imaging of Intact Proteins in Tissues.Analytical chemistry · 2026Article
- Quantitative mass spectrometry imaging: therapeutics & biomolecules.Chemical communications (Cambridge, England) · 2024Review
- Surface-sampling mass spectrometry to study proteins and protein complexes.Essays in biochemistry · 2023Review
- On-tissue chemical derivatization in mass spectrometry imaging.Mass spectrometry reviews · 2022Review
- High-Throughput Nano-DESI Mass Spectrometry Imaging of Biological Tissues Using an Integrated Microfluidic Probe.Analytical chemistry · 2022Article
- Quantitative Characterization of Three Carbonic Anhydrase Inhibitors by LESA Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2022Article
- Key defatting tissue pretreatment protocol for enhanced MALDI MS Imaging of peptide biomarkers visualization in the castor beans and their attribution applications.Frontiers in plant science · 2022Article
- Quantitative Mass Spectrometry Imaging of Biological Systems.Annual review of physical chemistry · 2021Review
- Spatially resolved absolute quantitation in thin tissue by mass spectrometry.Analytical and bioanalytical chemistry · 2021Review
- In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates.Biochemical Society transactions · 2020Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Absolute quantification of proteins in tissue is important for numerous fields of study. Liquid chromatography-mass spectrometry (LC-MS) methods are the norm but typically involve lengthy sample preparation including tissue homogenization, which results in the loss of information relating to spatial distribution. Here, we propose liquid extraction surface analysis (LESA) mass spectrometry (MS) of stable isotope labeled mimetic tissue models for the spatially resolved quantification of intact ubiquitin in rat and mouse brain tissue. Measured ubiquitin concentrations are in agreement with values found in the literature. Images of rat and mouse brain tissue demonstrate spatial variation in the concentration of ubiquitin and demonstrate the utility of spatially resolved quantitative measurement of proteins in tissue. Although we have focused on ubiquitin, the method has the potential for broader application to the absolute quantitation of any endogenous protein or protein-based drug in tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.