Evidence map›Paper›PMID 31659914›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019

Dazl determines primordial follicle formation through the translational regulation of Tex14.

Roseanne Rosario, James H Crichton, Hazel L Stewart, Andrew J Childs, Ian R Adams, Richard A Anderson

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
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  4. Review
  5. Review
  6. Review
  7. Primordial Follicle Formation - Some Assembly Required.Current opinion in endocrine and metabolic research · 2021
    Article
  8. Review
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Roseanne RosarioMedical Research Council (MRC) Centre for Reproductive Health, Queens Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom.
James H CrichtonMRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
Hazel L StewartMedical Research Council (MRC) Centre for Reproductive Health, Queens Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom.
Andrew J ChildsDepartment of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London, United Kingdom.
Ian R AdamsMRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
Richard A AndersonMedical Research Council (MRC) Centre for Reproductive Health, Queens Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom.
MRC Centre for Reproductive Health · GBImperial College London · GBMRC Institute of Genetics and Molecular Medicine · GBWestern General Hospital · GB

Funding

Biotechnology and Biological Sciences Research Council BB/R015635/1Medical Research Council G1100357Medical Research Council MC_UU_00007/6Medical Research Council MR/N022556/1
6 · The paper itself

Abstract

Deleted in azoospermia-like (DAZL) is a germ cell RNA-binding protein that is essential for entry and progression through meiosis. The phenotype of the Dazl knockout mouse has extensive germ cell loss because of incomplete meiosis. We have created a Dazl hypomorph model using short interfering RNA knockdown in mouse fetal ovary cultures, allowing investigation of Dazl function in germ cell maturation. Dazl hypomorph ovaries had a phenotype of impaired germ cell nest breakdown with a 66% reduction in total follicle number and an increase in the proportion of primordial follicles (PMFs), with smaller oocytes within these follicles. There was no significant early germ cell loss or meiotic delay. Immunostaining of intercellular bridge component testis-expressed protein (Tex)14 showed ∼59% reduction in foci number and size, without any change in Tex14 mRNA levels. TEX14 expression was also confirmed in the human fetal ovary across gestation. Using 3'UTR-luciferase reporter assays, translational regulation of TEX14 was demonstrated to be DAZL-dependant. Dazl is therefore essential for normal intercellular bridges within germ cell nests and their timely breakdown, with a major impact on subsequent assembly of PMFs.-Rosario, R., Crichton, J. H., Stewart, H. L., Childs, A. J., Adams, I. R., Anderson, R. A. Dazl determines primordial follicle formation through the translational regulation of Tex14.

Indexed as

AnimalsCloning, MolecularFemaleGene Expression Regulation, DevelopmentalHumansMeiosisMiceOvaryRNA-Binding ProteinsRNA InterferenceRNA, MessengerTissue Culture TechniquesTranscription FactorsDAZL protein, humanDAZL protein, mouseRNA-Binding ProteinsRNA, MessengerTEX14 protein, humanTEX14 protein, mouseTranscription Factorsfetal ovary culturegerm cell maturationintercellular bridgesiRNA knockdown

Identifiers

PMID31659914
PMCPMC6894055
OpenAlexW2982478343

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.