ArticlePLoS genetics2019
The quorum sensing transcription factor AphA directly regulates natural competence in Vibrio cholerae.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A 3'UTR-derived small RNA modulates the life cycle of the cholera toxin-encoding filamentous phage, CTXϕ.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Activation of bacterial transcription by distortion of promoter base pairing.Nucleic acids research · 2026Article
- Epigenetic regulation and antimicrobial resistance: functional roles of DNA methylation.FEMS microbiology reviews · 2026Review
- Quorum sensing regulators AphA and HapR differentially regulate fluvibactin biosynthesis inFrontiers in microbiology · 2026Article
- Parallel regulatory circuits orchestrate biofilm formation in response to c-di-GMP levels and growth phase.PLoS genetics · 2025Article
- Coordination of cell envelope biology by Escherichia coli MarA protein potentiates intrinsic antibiotic resistance.PLoS genetics · 2025Article
- H-NS is a bacterial transposon capture protein.Nature communications · 2024Article
- ChimericFragments: computation, analysis and visualization of global RNA networks.NAR genomics and bioinformatics · 2024Article
- Diverse regulatory pathways modulate bet hedging of competence induction in epigenetically-differentiated phase variants of Streptococcus pneumoniae.Nucleic acids research · 2023Article
- Perturbation of Quorum Sensing after the Acquisition of Bacteriophage Resistance Could Contribute to Novel Traits inMicroorganisms · 2023Article
- Article
- A simple mechanism for integration of quorum sensing and cAMP signalling inbioRxiv : the preprint server for biology · 2023Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Signal Transduction Network Principles Underlying Bacterial Collective Behaviors.Annual review of microbiology · 2022Review
- Expression of the AHPND Toxins PirAInternational journal of molecular sciences · 2022Article
- Genome-wide mapping of Vibrio cholerae VpsT binding identifies a mechanism for c-di-GMP homeostasis.Nucleic acids research · 2022Article
- Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival inCells · 2021Article
- Deficiency in cytosine DNA methylation leads to high chaperonin expression and tolerance to aminoglycosides in Vibrio cholerae.PLoS genetics · 2021Article
- Intraspecies heterogeneity in microbial interactions.Current opinion in microbiology · 2021Review
- Widespread divergent transcription from bacterial and archaeal promoters is a consequence of DNA-sequence symmetry.Nature microbiology · 2021Article
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7 authors.
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Abstract
Many bacteria use population density to control gene expression via quorum sensing. In Vibrio cholerae, quorum sensing coordinates virulence, biofilm formation, and DNA uptake by natural competence. The transcription factors AphA and HapR, expressed at low and high cell density respectively, play a key role. In particular, AphA triggers the entire virulence cascade upon host colonisation. In this work we have mapped genome-wide DNA binding by AphA. We show that AphA is versatile, exhibiting distinct modes of DNA binding and promoter regulation. Unexpectedly, whilst HapR is known to induce natural competence, we demonstrate that AphA also intervenes. Most notably, AphA is a direct repressor of tfoX, the master activator of competence. Hence, production of AphA markedly suppressed DNA uptake; an effect largely circumvented by ectopic expression of tfoX. Our observations suggest dual regulation of competence. At low cell density AphA is a master repressor whilst HapR activates the process at high cell density. Thus, we provide deep mechanistic insight into the role of AphA and highlight how V. cholerae utilises this regulator for diverse purposes.
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