ArticleJournal of occupational health2020
Multiple exposures to poultry barn air and lipopolysaccharide synergistically increase the pulmonary expression of TLR-4 and IL-1β.
Article in Journal of occupational health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- Detection and characterization of Campylobacter in air samples from poultry houses using shot-gun metagenomics - a pilot study.BMC microbiology · 2024Article
- Cervical cancer in low and middle-income countries.Oncology letters · 2020Review
- Multiple exposures to poultry barn air and lipopolysaccharide synergistically increase the pulmonary expression of TLR-4 and IL-1β.Journal of occupational health · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivePoultry farm workers are exposed to barn air and suffer from various respiratory disorders. Due to frequent prevalence of endotoxin in the farm settings workers can get co-exposed to barn air and endotoxin. The study was aimed to explore the pulmonary damage following long-term multiple exposures to poultry barn air with or without endotoxin.
methodsWe studied the pulmonary expression of Toll-like receptor 4 (TLR4) and Interleukin-1β (IL-1β) by exposing Swiss albino mice to poultry barn air for 6 days (Monday-Saturday) in a week for 5 and 10 weeks. At the end of exposure, animals were challenged with lipopolysaccharide (LPS) or normal saline solution @80 μg/mouse intranasally. Histopathology, bronchoalveolar lavage (BAL) fluid and blood analysis were used to characterize lung damage. mRNA and protein expression of TLR4 and IL-1β were evaluated using quantitative polymerase chain reaction (qPCR) and immunohistochemistry, respectively.
resultsHistopathology along with TLC and DLC of blood and BAL fluid revealed lung damage following multiple exposures and damage was severe in combination with LPS. Exposures altered mRNA and protein expression of TLR-4 and IL-1β and the expression was more marked following 30 days of exposure. Further LPS co-challenge showed a synergistic effect on the expression of TLR4 and IL-1β.
conclusionsThe data suggest that long-term exposures with or without LPS caused lung damage and altered the pulmonary expression of TLR4 and IL-1β.
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