Evidence map›Paper›PMID 31656549›Full record

ArticleThe journal of physical chemistry. C, Nanomaterials and interfaces2018

Structural Contributions to Hydrodynamic Diameter for Quantum Dots Optimized for Live-Cell Single-Molecule Tracking.

Janet Y Sheung, Pinghua Ge, Sung Jun Lim, Sang Hak Lee, Andrew M Smith, Paul R Selvin

Abstract read
In one paragraph

Article in The journal of physical chemistry. C, Nanomaterials and interfaces, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Janet Y SheungDepartment of Physics, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.ORCID 0000-0003-4694-9740
Pinghua GeDepartment of Physics, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
Sung Jun LimDepartment of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
Sang Hak LeeDepartment of Physics, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
Andrew M SmithDepartment of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
Paul R SelvinDepartment of Physics, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.

Funding

Super-Resolution Microscopy of Neuronal Synapses with Small Quantum Dots and Advanced Imaging ToolsR01NS100019 · NINDS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI CHUNG, HEE JUNG, SELVIN, PAUL R · 2017 to 2025
$3.2M
Measuring the Opening of the Mechanosensitive Channel through smFRET & MolecularR01GM108578 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI SELVIN, PAUL R · 2014 to 2017
$1.2M
NIGMS NIH HHS R01 GM108578NINDS NIH HHS R01 NS100019
6 · The paper itself

Abstract

Quantum dots are fluorescent nanoparticles with narrow-band, size-tunable, and long-lasting emission. Typical formulations used for imaging proteins in cells are hydrodynamically much larger than the protein targets, so it is critical to assess the impact of steric effects deriving from hydrodynamic size. This report analyzes a new class of quantum dots that have been engineered for minimized size specifically for imaging receptors in narrow synaptic junctions between neurons. We use fluorescence correlation spectroscopy and transmission electron microscopy to calculate the contributions of the crystalline core, organic coating, and targeting proteins (streptavidin) to the total hydrodynamic diameter of the probe, using a wide range of core materials with emission spanning 545-705 nm. We find the contributing thickness of standard commercial amphiphilic polymers to be ~8 to ~14 nm, whereas coatings based on the compact ligand HS-(CH

Identifiers

PMID31656549
PMCPMC6814160

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.