ArticleThe ocular surface2020
Investigation of factors associated with ABCB5-positive limbal stem cell isolation yields from human donors.
Article in The ocular surface, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- YAP1-dependent regulation of cell size in BCAM-positive limbal corneal progenitor cells.Experimental eye research · 2026Article
- The human limbus functions as an instructive epithelial transition zone.Research square · 2026Article
- Essential Role of CD63 in Maintaining Corneal Epithelial Identity in the Human Limbus.Investigative ophthalmology & visual science · 2026Article
- Characterization of LGR5 as a Definitive and Functional Marker for Human Limbal Epithelial Stem Cells.Investigative ophthalmology & visual science · 2025Article
- MYEOV Is a Novel Marker of Differentiated Corneal Epithelium.Investigative ophthalmology & visual science · 2025Article
- Caveolin 1 and 2 enhance the proliferative capacity of BCAM-positive corneal progenitors.Scientific reports · 2025Article
- Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.Cell reports · 2022Article
- Current and Emerging Therapies for Limbal Stem Cell Deficiency.Stem cells translational medicine · 2022Article
- High expression of SARS-CoV2 viral entry-related proteins in human limbal stem cells.The ocular surface · 2022Article
- Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities.iScience · 2021Article
- Process development and safety evaluation of ABCB5Stem cell research & therapy · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
purposeTo identify factors associated with isolation yields of ATP-binding cassette (ABC) superfamily member B5 (ABCB5)-positive limbal stem cells (LSCs) from human cadaveric donor eyes.
methodsWhole eye globes were obtained from the Saving Sight eye bank, Kansas City, MO and the CorneaGen eye bank, Seattle, WA. ABCB5-positive LSCs were sorted by flow cytometry upon anti-ABCB5 monoclonal antibody staining within one week after donor death. The yields of live limbal epithelial cells in their entirety and of isolated pure ABCB5-positive LSC subsets were correlated with variables contained in the eye donors' medical information.
resultsThe mean isolation yield of live limbal epithelial cells and ABCB5-positive LSCs per donor eye was (340,000 ± 160,000 and 2,608 ± 1,842 respectively, mean ± SD). Stepwise regression analysis showed that cardiac disease-related death was the strongest negative predictor of the ABCB5-positive LSC isolation yield (p = 0.01). While we observed a trend for an age-related decline in the yield of ABCB5-positive LSCs, a statistically significant association could not be established (2% decrease/year, p = 0.11). Additionally, despite a trend for decreased isolation yields of total live limbal epithelial cells isolated from single donors with a longer time between death and tissue processing (p = 0.04), this did not affect the yields of purified ABCB5-positive LSC, which was independent of increasing time between death and tissue processing (p = 0.50).
conclusionsOur study identifies cardiac disease-related death as a donor variable significantly associated with lower ABCB5-positive LSC isolation yields.
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