Evidence map›Paper›PMID 31654340›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2020

A novel method for rapid and sensitive detection of viable Escherichia coli cells using UV-induced PMA-coupled quantitative PCR.

Rehan Deshmukh, Sunil Bhand, Utpal Roy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 16 citations in OpenAlex.

  1. Quantitative Detection of Viable but NonculturableFoods (Basel, Switzerland) · 2022
    Article
  2. Article
  3. A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable UropathogenicFrontiers in cellular and infection microbiology · 2022
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rehan DeshmukhDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani, Goa Campus, NH17B Bypass, Goa, 403726, India.
Sunil BhandDepartment of Chemistry, Birla Institute of Technology and Science, Pilani, Goa Campus, NH17B Bypass, Goa, 403726, India.
Utpal RoyDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani, Goa Campus, NH17B Bypass, Goa, 403726, India. utpalroy@gmail.com.ORCID http://orcid.org/0000-0001-6315-4523
Birla Institute of Technology and Science, Pilani - Goa Campus · IN

Funding

Birla Institute of Technology and Science, Pilani CORE-WWEM
6 · The paper itself

Abstract

We report a specific and sensitive method to improve the coupling of propidium monoazide (PMA) with DNA derived from killed cells of Escherichia coli using UV light of 365 nm. UV light of three different intensities mainly 2.4 × 10

Indexed as

Ultraviolet RaysAzidesCross-Linking ReagentsDNA, BacterialEscherichia coliHot TemperatureIndicators and ReagentsMicrobial ViabilityPropidiumReal-Time Polymerase Chain ReactionSensitivity and SpecificityWater MicrobiologyAzidesCross-Linking ReagentsDNA, BacterialIndicators and ReagentsPropidiumpropidium monoazideEscherichia coliMonitoringPropidium monoazideqPCRUltravioletViable

Identifiers

PMID31654340
PMCPMC7203400
OpenAlexW2982080045

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.