ArticleFunctional & integrative genomics2020
Evaluation of circulating leukocyte transcriptome and its relationship with immune function and blood markers in dairy cows during the transition period.
Article in Functional & integrative genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- Gene Expression in Circulating Leukocytes in Brown Swiss and Holstein Cows During the Transition Period.Animals : an open access journal from MDPI · 2026Article
- Maternal Hydroxytyrosol Supplementation Enhances Antioxidant Capacity and Immunometabolic Adaptations in Nutrient-Restricted Beef Cows and Their Offspring.Antioxidants (Basel, Switzerland) · 2025Article
- Investigating mitochondrial uncoupling protein 1 and leptin in the interplay of metabolic adaptation and inflammatory response of dairy cows during the peripartum period.Frontiers in veterinary science · 2025Article
- Genome-Wide Association Study of Conformation Traits in Brazilian Holstein Cattle.Animals : an open access journal from MDPI · 2024Article
- Scientific characterization methods for better utilization of cattle dung and urine: a concise review.Tropical animal health and production · 2023Review
- Integrating on-farm and genomic information improves the predictive ability of milk infrared prediction of blood indicators of metabolic disorders in dairy cows.Genetics, selection, evolution : GSE · 2023Article
- Medium chain fatty acid supplementation improves animal metabolic and immune status during the transition period: A study on dairy cattle.Frontiers in immunology · 2023Article
- Changes of acute-phase proteins, glucose, and lipid metabolism during pregnancy in lactating dairy cows.Archives animal breeding · 2022Article
- Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes.BMC genomics · 2021Article
- Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off.PloS one · 2021Article
- Interaction between inflammation and metabolism in periparturient dairy cows.Journal of animal science · 2020Article
- The Role of Innate Immune Response and Microbiome in Resilience of Dairy Cattle to Disease: The Mastitis Model.Animals : an open access journal from MDPI · 2020Review
- Use of gene expression profile to identify potentially relevant transcripts to myofibrillar fragmentation index trait.Functional & integrative genomics · 2020Article
- Mismatch of Glucose Allocation between Different Life Functions in the Transition Period of Dairy Cows.Animals : an open access journal from MDPI · 2020Review
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dairy cows during the transition period are faced with important physiological changes which include a dysfunctional immune system and an increased inflammatory state. New data are necessary to understand the key factors involved in the immune system regulation. Six dairy cows were sampled during transition period to investigate the leukocyte transcriptome changes and its relationship with blood biomarkers. Blood samples were collected at - 20 ± 2, - 3 ± 1, 3, and 7 days from parturition (DFP). Leukocyte transcriptome was analyzed by deep sequencing technology (Hiseq1000 Illumina, USA). Plasma was analyzed for metabolic biomarkers. Differentially expressed genes (DEG) were used to run an enrichment analysis through the Dynamic Impact Approach (DIA). Considering - 20 DFP as references time, the main KEGG impacted pathways were activated before calving (- 3 DFP) and were connected to lipid metabolism, lipid transport in plasma, and phagosome. The greatest differences were found after parturition with 281 DEG (179 upregulated and 102 downregulated). The activated pathways were mainly related to immunity and endocrine aspects, while metabolic pathways related to lipid and amino acid metabolism were inhibited. Plasma BHBA had a substantial inhibitory impact on KEGG pathways related to DNA replication and cell cycle, while plasma IL-1β had an inhibitory impact on fatty acid elongation in mitochondria and an activated impact in several pathways related to cellular energy metabolism. Overall, this study confirmed that many changes in lipid metabolism and immune competence of the circulating leukocytes occurred in dairy cow around calving. Interestingly, BHBA and IL-1β connected with the transcriptome.
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