Evidence map›Paper›PMID 31654133›Full record

ArticleMikrochimica acta2019

Graphene oxide and enzyme-assisted dual-cycling amplification method for sensitive fluorometric determination of DNA.

Idorenyin Iwe, Zhigang Li, Jiahao Huang

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. 2'-RSC advances · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Idorenyin IweDepartment of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Zhigang LiDepartment of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. mezli@ust.hk.
Jiahao HuangGuangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515, China. jhuangaf@connect.ust.hk.ORCID http://orcid.org/0000-0002-6190-456X
Hong Kong University of Science and Technology · HKSouthern Medical University · CN

Funding

National Natural Science Foundation of China 21705076Research Grants Council of the Hong Kong Special Administrative Region 16228216
6 · The paper itself

Abstract

A fluorometric method is described for the determination of DNA. It involves the use of graphene oxide (GO), exonuclease III (Exo III), and two specially designed fluorophore-labeled hairpin probes (HP1 and HP2). Different from other GO-based DNA assays, the method takes advantage of the distinct binding abilities of GO with hairpin DNA probes and single nucleotides. GO serves as a strong quencher for fluorescent labels to ensure a very low background signal. Two reaction cycles mediated by Exo III are employed to enhance the signals. The combination of GO-induced quenching and Exo III-mediated dual regeneration of analytes leads to a detection limit as low as 1 pM for the model analyte human hemochromatosis protein (HFE) gene. The method is also applicable for the determination of HFE gene spiked into fetal bovine serum. Graphical abstract Schematic representation of a GO-based, Exo III-assisted method for dual-signal amplified detection of DNA, for which human haemochromatosis protein (HFE) gene is designed as the model target. The assay involves graphene oxide (GO), exonuclease (Exo III), and two specially designed, fluorophore-labelled hairpin probes (HP1 and HP2).

Indexed as

AnimalsCattleDNADNA ProbesExodeoxyribonucleasesFluoresceinsFluorescent DyesGraphiteInverted Repeat SequencesLimit of DetectionNucleic Acid HybridizationSpectrometry, FluorescenceDNADNA Probesexodeoxyribonuclease IIIExodeoxyribonucleasesFluoresceinsFluorescent Dyesgraphene oxideGraphiteDNA sensorExonuclease IIIFetal bovine serumFluorescenceHuman hemochromatosis protein geneNanomaterial

Identifiers

PMID31654133
OpenAlexW2982180644

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.