ArticleScientific reports2019
Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Optimization of Conditions for Cryopreservation of Enriched Spermatogonial Stem Cells in Olive Flounder (Cells · 2026Article
- Conservation of Teleost Fishes: Current Status and the Role of Reproductive Technologies.Advances in experimental medicine and biology · 2026Review
- Cryopreservation toxicity and morphological outcomes inAnimal reproduction · 2026Article
- Retrospect and prospect: reproductive technologies in beef cattle.Molecular biology reports · 2025Review
- Alterations in Gene Expression and the Fatty Acid Profile Impact but Do Not Compromise the In Vitro Maturation of Zebrafish (Animals : an open access journal from MDPI · 2023Article
- PPAR Alpha Activation by Clofibrate Alleviates Ischemia/Reperfusion Injury in Metabolic Syndrome Rats by Decreasing Cardiac Inflammation and Remodeling and by Regulating the Atrial Natriuretic Peptide Compensatory Response.International journal of molecular sciences · 2023Article
- Rediscovery of poly(ethylene glycol)s as a cryoprotectant for mesenchymal stem cells.Biomaterials research · 2023Article
- Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.Bioengineering (Basel, Switzerland) · 2022Review
- The Protective Role of Melatonin in Sperm Cryopreservation of Farm Animals and Human: Lessons for Male Fish Cryopreservation.Animals : an open access journal from MDPI · 2022Review
- Cryopreservation of Testicular Tissue from Adult Red-Rumped Agoutis (Animals : an open access journal from MDPI · 2022Article
- Ultra-Rapid Laser Calorimetry for the Assessment of Crystallization in Low-Concentration Cryoprotectants.Journal of heat transfer · 2022Article
- Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges.Animals : an open access journal from MDPI · 2022Review
- Role of Klotho as a Modulator of Oxidative Stress Associated with Ovarian Tissue Cryopreservation.International journal of molecular sciences · 2021Article
- Larval cryopreservation as new management tool for threatened clam fisheries.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the best cryoprotectant solution for each cryopreservation method. Primary growth (PG) oocytes showed the best percentage of membrane integrity (63.5 ± 2.99%) when SF4 solution (2 M methanol + 0.1 M trehalose + 10% egg yolk solution) was employed. The vitrification solution, which presented the highest membrane integrity (V2; 1.5 M methanol + 5.5 M Me
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