Evidence map›Paper›PMID 31651088›Full record

ArticleMicrobial biotechnology2020

Determination of live and dead Komagataeibacter xylinus cells and first attempt at precise control of inoculation in nanocellulose production.

Xiaozhou Zou, Shuo Zhang, Lin Chen, Junqing Hu, Feng F Hong

Open access · goldAbstract read
In one paragraph

Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xiaozhou ZouKey Lab of Science and Technology of Eco-textile, Ministry of Education, Donghua University, Shanghai, China.
Shuo ZhangCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, North Renmin Road 2999, Shanghai, 201620, China.
Lin ChenCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, North Renmin Road 2999, Shanghai, 201620, China.
Junqing HuState Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, China.
Feng F HongKey Lab of Science and Technology of Eco-textile, Ministry of Education, Donghua University, Shanghai, China.ORCID 0000-0001-5120-3519
Donghua University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The timely enumeration of cells of nanocellulose-producing bacteria is challenging due to their unique growth properties. To better understand the metabolism of the bacteria and better control the concentration of living cells during cultivation, a prompt cell counting technology is crucial and urgently required. In this work, two fluorescent dyes, the asymmetrical anthocyanidin dye SYBR Green I (SG) and propidium iodide (PI), were first combined for Komagataeibacter xylinus species to determine live/dead bacterial cells quantitatively and promptly. The number of live and dead K. xylinus cells determined using an epifluorescence microscope corresponded well to the results obtained using a fluorescence microplate reader. The R

Indexed as

Gluconacetobacter xylinusBacteriaCelluloseFluorescent DyesStaining and LabelingCelluloseFluorescent Dyes

Identifiers

PMID31651088
PMCPMC7017834
OpenAlexW2981322903

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.