Evidence map›Paper›PMID 31650483›Full record

ArticleMolecular imaging and biology2020

Simple and Robust Intravital Microscopy Procedures in Hybrid TIE2GFP-BALB/c Transgenic Mice.

Alexandros Marios Sofias, Andreas K O Åslund, Nils Hagen, Kristin Grendstad, Sjoerd Hak

Abstract read
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In one paragraph

Article in Molecular imaging and biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Tumor Targeting by αACS nano · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Alexandros Marios SofiasDepartment of Circulation and Medical Imaging, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway. alexandrosofias@outlook.com.ORCID http://orcid.org/0000-0002-7475-2526
Andreas K O ÅslundDepartment of Physics, Faculty of Natural Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Nils HagenComparative Medicine Core Facility, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Kristin GrendstadDepartment of Physics, Faculty of Natural Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Sjoerd HakDepartment of Circulation and Medical Imaging, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway. sjoerd.hak@ntnu.no.
Norwegian University of Science and Technology · NOSINTEF · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe endeavor of deciphering intricate phenomena within the field of molecular medicine dictates the necessity to investigate tumor/disease microenvironment real-time on cellular level. We, hereby, design simple and robust intravital microscopy strategies, which can be used to elucidate cellular or molecular interactions in a fluorescent mouse model. PROCEDURES: We crossbred transgenic TIE2GFP mice with nude BALB/c mice, allowing the breeding of immunocompetent and immunodeficient mouse models expressing green fluorescent protein (GFP) in vascular endothelium. Then, we surgically exposed various tissues of interest to perform intravital microscopy.

resultsBy utilizing simple tissue preparation procedures and confocal or two-photon microscopy, we produced high-resolution static snapshots, dynamic sequences, and 3D reconstructions of orthotopically grown mammary tumor, skin inflammation, brain, and muscle. The homogenous detection of GFP expressed by endothelial cells and a combination of fluorescence agents enabled landmarking of tumor microenvironment and precise molecular tagging.

conclusionSimple intravital microscopy procedures on TIE2GFP mice allowed a real-time multi-color visualization of tissue microenvironment, underlining that robust microscopy strategies are relatively simple and can be readily available for many tissues of interest.

Indexed as

AnimalsBreast NeoplasmsCell Line, TumorDisease Models, AnimalEndothelial CellsFemaleGreen Fluorescent ProteinsIntravital MicroscopyMiceMice, Inbred BALB CMice, NudeMice, TransgenicMicroscopy, ConfocalReceptor, TIE-2Tumor MicroenvironmentGreen Fluorescent ProteinsReceptor, TIE-2Tek protein, mouseConfocal microscopyFluorescenceIntravital microscopyMolecular imagingMultiphoton microscopyOptical imagingTransgenic TIE2GFP mouse

Identifiers

PMID31650483
OpenAlexW2981372428

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.