ArticleFrontiers in bioengineering and biotechnology2019
Oxygen Consumption Characteristics in 3D Constructs Depend on Cell Density.
Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Recent advancements and future requirements in vascularization of cortical organoids.Frontiers in bioengineering and biotechnology · 2022Pooled it
- Advances in vascularized organoids.Chinese medical journal · 2026Review
- Computational Assessment of Oxygen Availability and Shear Stress in Microfluidic Cell Culture Chambers for Optimized Cell Adhesion.Biosensors · 2026Article
- Computational design of artificial supply networks for engineered human tissue.Scientific reports · 2026Article
- Modeling of Biomechanical and Functional Parameters of Hydrogel-Cell Composites Fabricated by 3D Bioprinting Using AI-Supported Approach.Materials (Basel, Switzerland) · 2026Article
- Hypoxia-induced drug-resistance bias 3D cancer spheroid drug screens.APL bioengineering · 2026Article
- Nanobubble-Mediated Oxygen Delivery Mitigates Hypoxia-Induced ROS and HIF-1α Expression in UC-MSCs.Nanomaterials (Basel, Switzerland) · 2026Article
- Integration of multi-modal monitoring for dynamic control of large-scale 3D tissue bioreactors.Scientific reports · 2026Article
- A feedback loop between cell proliferation and ROS regulates ferroptosis sensitivity.Frontiers in cell and developmental biology · 2026Article
- Pericellular oxygen dynamics in human cardiac fibroblasts and iPSC-cardiomyocytes in high-throughput plates: insights from experiments and modeling.Journal of molecular and cellular cardiology plus · 2025Article
- Oxygenating respiratoid biosystem for therapeutic cell transplantation.Nature communications · 2024Article
- Size-related variability of oxygen consumption rates in individual human hepatic cells.Lab on a chip · 2024Article
- Controlled oxygen delivery to power tissue regeneration.Nature communications · 2024Review
- Development of a novel high-throughput culture system for hypoxic 3D hydrogel cell culture.Scientific reports · 2024Article
- Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging.Npj imaging · 2024Article
- Integrated Experimental and Mathematical Exploration of Modular Tissue Cultures for Developmental Engineering.International journal of molecular sciences · 2024Article
- Three-DimensionalInternational journal of biological sciences · 2024Review
- Dragging 3D printing technique controls pore sizes of tissue engineered blood vessels to induce spontaneous cellular assembly.Bioactive materials · 2024Article
- NanoBubble-Mediated Oxygenation: Elucidating the Underlying Molecular Mechanisms in Hypoxia and Mitochondrial-Related Pathologies.Nanomaterials (Basel, Switzerland) · 2023Review
- Mathematical modelling with Bayesian inference to quantitatively characterize therapeutic cell behaviour in nerve tissue engineering.Journal of the Royal Society, Interface · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxygen is not only crucial for cell survival but also a determinant for cell fate and function. However, the supply of oxygen and other nutrients as well as the removal of toxic waste products often limit cell viability in 3-dimensional (3D) engineered tissues. The aim of this study was to determine the oxygen consumption characteristics of 3D constructs as a function of their cell density. The oxygen concentration was measured at the base of hepatocyte laden constructs and a tightly controlled experimental and analytical framework was used to reduce the system geometry to a single coordinate and enable the precise identification of initial and boundary conditions. Then dynamic process modeling was used to fit the measured oxygen vs. time profiles to a reaction and diffusion model. We show that oxygen consumption rates are well-described by Michaelis-Menten kinetics. However, the reaction parameters are not literature constants but depend on the cell density. Moreover, the average cellular oxygen consumption rate (or OCR) also varies with density. We discuss why the OCR of cells is often misinterpreted and erroneously reported, particularly in the case of 3D tissues and scaffolds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.