ArticleeLife2019
Extraction of active RhoGTPases by RhoGDI regulates spatiotemporal patterning of RhoGTPases.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Non-canonical and constitutive activation of small GTPases: more than an exception to the rule?The Biochemical journal · 2026Review
- Sortase A-Mediated Farnesylation of Cdc42ACS synthetic biology · 2026Article
- Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP.EMBO reports · 2026Article
- Distinct Cdc42 protein levels differentially regulate polarized growth and cell fusion in Schizosaccharomyces pombe.PLoS biology · 2026Article
- Anillin mediates unilateral furrowing during cytokinesis by limiting RhoA binding to its effectors.The Journal of cell biology · 2025Article
- The biochemical mechanism of Rho GTPase membrane binding, activation and retention in activity patterning.The EMBO journal · 2025Article
- Effects of fluorescent tags and activity status on the membrane localization of ROP GTPases.Plant signaling & behavior · 2024Article
- Synthesis and in vitro evaluation of benzo[b]thiophene-3-carboxylic acid 1,1-dioxide derivatives as anticancer agents targeting the RhoA/ROCK pathway.Journal of enzyme inhibition and medicinal chemistry · 2024Article
- RhoGDI in RBL-2H3 cells acts as a negative regulator of Rho GTPase signaling to inhibit granule exocytosis.Journal of leukocyte biology · 2024Article
- Transient Structural Properties of the Rho GDP-Dissociation Inhibitor.Angewandte Chemie (International ed. in English) · 2024Article
- The ion channel Anoctamin 10/TMEM16K coordinates organ morphogenesis across scales in the urochordate notochord.PLoS biology · 2024Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Atypical RhoUV GTPases in development and disease.Biochemical Society transactions · 2024Review
- RhoGDI1 regulates cell-cell junctions in polarized epithelial cells.Frontiers in cell and developmental biology · 2024Article
- The Rho guanine dissociation inhibitor α inhibits skeletal muscle Rac1 activity and insulin action.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Scar/WAVE has Rac GTPase-independent functions during cell wound repair.Scientific reports · 2023Article
- Coordinated efforts of different actin filament populations are needed for optimal cell wound repair.Molecular biology of the cell · 2023Article
- ELMOD3-Rab1A-Flotillin2 cascade regulates lumen formation via vesicle trafficking inOpen biology · 2023Article
- Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression.ACS synthetic biology · 2023Article
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Abstract
The RhoGTPases are characterized as membrane-associated molecular switches that cycle between active, GTP-bound and inactive, GDP-bound states. However, 90-95% of RhoGTPases are maintained in a soluble form by RhoGDI, which is generally viewed as a passive shuttle for inactive RhoGTPases. Our current understanding of RhoGTPase:RhoGDI dynamics has been limited by two experimental challenges: direct visualization of the RhoGTPases in vivo and reconstitution of the cycle in vitro. We developed methods to directly image vertebrate RhoGTPases in vivo or on lipid bilayers in vitro. Using these methods, we identified pools of active and inactive RhoGTPase associated with the membrane, found that RhoGDI can extract both inactive and active RhoGTPases, and found that extraction of active RhoGTPase contributes to their spatial regulation around cell wounds. These results indicate that RhoGDI directly contributes to the spatiotemporal patterning of RhoGTPases by removing active RhoGTPases from the plasma membrane.
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