Evidence map›Paper›PMID 31646861›Full record

ArticleAnalytical chemistry2019

Isolating Rare Cells and Circulating Tumor Cells with High Purity by Sequential eDAR.

Eleanor S Johnson, Shihan Xu, Hui-Min Yu, Wei-Feng Fang, Yuling Qin, Li Wu, Jiasi Wang, Mengxia Zhao, Perry G Schiro, Bryant Fujimoto and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Chemical science · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Eleanor S JohnsonDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.
Shihan XuDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.ORCID 0000-0002-0938-6902
Hui-Min YuMiCareo Inc. , Xing-Ai Road Ln. 77 No. 69 5F , Taipei City , Taiwan.
Wei-Feng FangMiCareo Inc. , Xing-Ai Road Ln. 77 No. 69 5F , Taipei City , Taiwan.
Yuling QinDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.
Li WuDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.ORCID 0000-0002-4118-7970
Jiasi WangDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.ORCID 0000-0003-4619-6814
Mengxia ZhaoDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.
Perry G SchiroMiCareo Inc. , Xing-Ai Road Ln. 77 No. 69 5F , Taipei City , Taiwan.
Bryant FujimotoDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.
Jui-Lin ChenMiCareo Inc. , Xing-Ai Road Ln. 77 No. 69 5F , Taipei City , Taiwan.
Daniel T ChiuDepartment of Chemistry , University of Washington , Box 351700, Seattle , Washington , United States.ORCID 0000-0003-2964-9578

Funding

Placentomics using a novel method to isolate circulating placental derivativesR01HD089679 · NICHD · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T, GRAVETT, MICHAEL G · 2016 to 2020
$3.1M
NICHD NIH HHS R01 HD089679
6 · The paper itself

Abstract

Isolation and analysis of circulating tumor cells (CTCs) from the blood of patients at risk of metastatic cancers is a promising approach to improving cancer treatment. However, CTC isolation is difficult due to low CTC abundance and heterogeneity. Previously, we reported an ensemble-decision aliquot ranking (eDAR) platform for the rare cell and CTC isolation with high throughput, greater than 90% recovery, and high sensitivity, allowing detection of low surface antigen-expressing cells linked to metastasis. Here we demonstrate a sequential eDAR platform capable of isolating rare cells from whole blood with high purity. This improvement in purity is achieved by using a sequential sorting and flow stretching design in which whole blood is sorted and fluid elements are stretched using herringbone features and the parabolic flow profile being sorted a second time. This platform can be used to collect single CTCs in a multiwell plate for downstream analysis.

Indexed as

Blood CellsNeoplastic Cells, CirculatingCell SeparationHumansLab-On-A-Chip DevicesMCF-7 CellsMicrofluidic Analytical Techniques

Identifiers

PMID31646861
PMCPMC9847251

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.