ArticleScientific reports2019
A versatile microfluidic device for highly inclined thin illumination microscopy in the moss Physcomitrella patens.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Microfluidic Interrogation of Chitin-Induced Calcium Oscillations in the MossPlants (Basel, Switzerland) · 2026Article
- FLUID-CELL: Flow-enabled Light and Ultrastructural Imaging Device for Correlative Electron and Light Localization.bioRxiv : the preprint server for biology · 2025Article
- Class II kinesin-12 facilitates cell plate formation by transporting cell plate materials in the phragmoplast.Nature plants · 2025Article
- CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes.Frontiers in plant science · 2025Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Development of a tomato xylem-mimicking microfluidic system to studyFrontiers in bioengineering and biotechnology · 2024Article
- Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species.Life science alliance · 2023Article
- Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization.Developmental cell · 2022Article
- Imaging plant cells and organs with light-sheet and super-resolution microscopy.Plant physiology · 2022Article
- Physcomitrium patens Protoplasting and Protoplast Transfection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Microfluidics-Based Bioassays and Imaging of Plant Cells.Plant & cell physiology · 2021Review
- Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the MossThe Plant cell · 2020Article
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2 authors.
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Abstract
High-resolution microscopy is a valuable tool for studying cellular processes, such as signalling, membrane trafficking, or cytoskeleton remodelling. Several techniques of inclined illumination microscopy allow imaging at a near single molecular level; however, the application of these methods to plant cells is limited, owing to thick cell walls as well as the necessity to excise a part of the tissue for sample preparation. In this study, we utilised a simple, easy-to-use microfluidic device for highly inclined and laminated optical sheet (HILO) microscopy using a model plant Physcomitrella patens. We demonstrated that the shallow microfluidic device can be used for long-term culture of living cells and enables high-resolution HILO imaging of microtubules without perturbing their dynamics. In addition, our microdevice allows the supply and robust washout of compounds during HILO microscopy imaging, for example, to perform a microtubule regrowth assay. Furthermore, we tested long-term (48 h) HILO imaging using a microdevice and visualised the developmental changes in the microtubule dynamics during tissue regeneration. These novel applications of the microfluidic device provide a valuable resource for studying molecular dynamics in living plant cells.
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