ArticleMolecular plant pathology2020
Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III-secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus.
Article in Molecular plant pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 41 citations in OpenAlex.
- Identification of protein secretion systems and type III effectors in wood-associated bacteria of the genusMicrobiology spectrum · 2026Article
- Phylogenomic analysis of Paracidovorax citrulli strains reveals the presence of two lineages in Brazil.Genetics and molecular biology · 2026Article
- Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12.Molecular plant pathology · 2025Article
- HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.Molecular plant pathology · 2025Article
- Effectidor II: a pan-genomic AI-based algorithm for the prediction of type III secretion system effectors.Bioinformatics (Oxford, England) · 2025Article
- Article
- Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Microscope Subcellular Localization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures.Methods in molecular biology (Clifton, N.J.) · 2024Article
- ClpA affects the virulence ofFrontiers in microbiology · 2024Article
- Differential plant cell responses tomBio · 2023Article
- Article
- Proteomic and Phenotypic Analyses of a Putative YggS Family Pyridoxal Phosphate-Dependent Enzyme in Acidovorax citrulli.The plant pathology journal · 2023Article
- Multitask Approach to Localize Rhizobial Type Three Secretion System Effector Proteins Inside Eukaryotic Cells.Plants (Basel, Switzerland) · 2023Article
- Complete genome sequence of an Israeli isolate ofFrontiers in plant science · 2023Article
- Article
- Article
- EssentialInternational journal of molecular sciences · 2022Article
- The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic BacteriumMicroorganisms · 2022Article
- Recent Advancements in Tracking Bacterial Effector Protein Translocation.Microorganisms · 2022Review
- A putative multi-sensor hybrid histidine kinase, BarAFrontiers in microbiology · 2022Article
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cucurbit pathogenic bacterium Acidovorax citrulli requires a functional type III secretion system (T3SS) for pathogenicity. In this bacterium, as with Xanthomonas and Ralstonia spp., an AraC-type transcriptional regulator, HrpX, regulates expression of genes encoding T3SS components and type III-secreted effectors (T3Es). The annotation of a sequenced A. citrulli strain revealed 11 T3E genes. Assuming that this could be an underestimation, we aimed to uncover the T3E arsenal of the A. citrulli model strain, M6. Thorough sequence analysis revealed 51 M6 genes whose products are similar to known T3Es. Furthermore, we combined machine learning and transcriptomics to identify novel T3Es. The machine-learning approach ranked all A. citrulli M6 genes according to their propensity to encode T3Es. RNA-Seq revealed differential gene expression between wild-type M6 and a mutant defective in HrpX: 159 and 28 genes showed significantly reduced and increased expression in the mutant relative to wild-type M6, respectively. Data combined from these approaches led to the identification of seven novel T3E candidates that were further validated using a T3SS-dependent translocation assay. These T3E genes encode hypothetical proteins that seem to be restricted to plant pathogenic Acidovorax species. Transient expression in Nicotiana benthamiana revealed that two of these T3Es localize to the cell nucleus and one interacts with the endoplasmic reticulum. This study places A. citrulli among the 'richest' bacterial pathogens in terms of T3E cargo. It also revealed novel T3Es that appear to be involved in the pathoadaptive evolution of plant pathogenic Acidovorax species.
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