ArticleNucleic acids research2019
Controlling aggregation of cholesterol-modified DNA nanostructures.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Programmable Lipid Functionalization of Nucleic Acid Nanoparticles Modulates Liver Cell-Type Targeting.ACS applied materials & interfaces · 2026Article
- Membrane-Penetrating Molecular Device Based on a Triplex Containing Acyclic L-Threoninol Nucleic Acid Functionalized with Cholesterol.Angewandte Chemie (International ed. in English) · 2026Article
- Bacteriophage-Mimetic DNA Origami Needle for Targeted Membrane Penetration and Cytosolic Cargo Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The effect of PEGylation on surface tethering of liposomes via DNA nanotechnology.Journal of lipid research · 2026Article
- Functionalizing Nucleic Acids: Synthesis and Purification Strategies for Bioconjugates as Biomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Hierarchy of Hydrophobic and Electrostatic Interactions in DNA-Membrane Phase Selectivity.ACS applied materials & interfaces · 2025Article
- Microswimmers That Flex: Advancing Microswimmers with Templated Assembly and Responsive DNA Nanostructures.Accounts of materials research · 2025Article
- Article
- DNA Origami Vesicle Sensors with Triggered Single-Molecule Cargo Transfer.Angewandte Chemie (International ed. in English) · 2024Article
- Modulating the DNA/Lipid Interface through Multivalent Hydrophobicity.Nano letters · 2024Article
- Cholesterol and Cytokines: Molecular Links to Atherosclerosis and Carcinogenesis.Cell biochemistry and biophysics · 2024Review
- DNA-empowered synthetic cells as minimalistic life forms.Nature reviews. Chemistry · 2024Review
- Barium Concentration-Dependent Anomalous Electrophoresis of Synthetic DNA Motifs.ACS applied bio materials · 2024Article
- Cell-Surface Binding of DNA Nanostructures for Enhanced Intracellular and Intranuclear Delivery.ACS applied materials & interfaces · 2024Article
- Nucleic acid amphiphiles: Synthesis, properties, and applications.Molecular therapy. Nucleic acids · 2023Review
- DNA nanopores as artificial membrane channels for bioprotonics.Nature communications · 2023Article
- Structure and dynamics of an archetypal DNA nanoarchitecture revealed via cryo-EM and molecular dynamics simulations.Nature communications · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
DNA nanotechnology allows for the design of programmable DNA-built nanodevices which controllably interact with biological membranes and even mimic the function of natural membrane proteins. Hydrophobic modifications, covalently linked to the DNA, are essential for targeted interfacing of DNA nanostructures with lipid membranes. However, these hydrophobic tags typically induce undesired aggregation eliminating structural control, the primary advantage of DNA nanotechnology. Here, we study the aggregation of cholesterol-modified DNA nanostructures using a combined approach of non-denaturing polyacrylamide gel electrophoresis, dynamic light scattering, confocal microscopy and atomistic molecular dynamics simulations. We show that the aggregation of cholesterol-tagged ssDNA is sequence-dependent, while for assembled DNA constructs, the number and position of the cholesterol tags are the dominating factors. Molecular dynamics simulations of cholesterol-modified ssDNA reveal that the nucleotides wrap around the hydrophobic moiety, shielding it from the environment. Utilizing this behavior, we demonstrate experimentally that the aggregation of cholesterol-modified DNA nanostructures can be controlled by the length of ssDNA overhangs positioned adjacent to the cholesterol. Our easy-to-implement method for tuning cholesterol-mediated aggregation allows for increased control and a closer structure-function relationship of membrane-interfacing DNA constructs - a fundamental prerequisite for employing DNA nanodevices in research and biomedicine.
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