Evidence map›Paper›PMID 31642042›Full record

ArticleMolecular biology reports2020

Chlorella vulgaris modulates the expression of senescence-associated genes in replicative senescence of human diploid fibroblasts.

Faizul Jaafar, Lina Wati Durani, Suzana Makpol

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Effect of Selected Antioxidants on the In Vitro Aging of Human Fibroblasts.International journal of molecular sciences · 2024
    Article
  2. Review
  3. Article
  4. Effect of Antioxidants on the Fibroblast Replicative LifespanOxidative medicine and cellular longevity · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Faizul JaafarDepartment of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia.
Lina Wati DuraniDepartment of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia.
Suzana MakpolDepartment of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia. suzanamakpol@ppukm.ukm.edu.my.ORCID http://orcid.org/0000-0002-5239-6196
University Kebangsaan Malaysia Medical Centre · MY

Funding

Malaysia Ministry of Education FRGS/2/2014/SKK01/UKM/01/1
6 · The paper itself

Abstract

Human diploid fibroblasts (HDFs) cultured in vitro have limited capacity to proliferate after population doubling is repeated several times, and they enter into a state known as replicative senescence or cellular senescence. This study aimed to investigate the effect of Chlorella vulgaris on the replicative senescence of HDFs by determining the expression of senescence-associated genes. Young and senescent HDFs were divided into untreated control and C. vulgaris-treated groups. A senescence-associated gene transcription analysis was carried out with qRT-PCR. Treatment of young HDFs with C. vulgaris reduced the expression of SOD1, CAT and CCS (p < 0.05). In addition, the expression of the SOD2 gene was increased with C. vulgaris treatment in young, pre-senescent and senescent HDFs (p < 0.05). Treatment of senescent HDFs with C. vulgaris resulted in the downregulation of TP53 gene expression. The expression of the CDKN2A gene was significantly decreased upon C. vulgaris treatment in young and senescent HDFs. C. vulgaris treatment was also found to significantly upregulate the expression of the MAPK14 gene in pre-senescent HDFs. In addition, the expression of MAPK14 was significantly upregulated compared to that in the untreated senescent HDFs (p < 0.05). In summary, the expression of senescence-associated genes related to antioxidants and the insulin/insulin-like growth factor-1 signalling, DNA damage-associated signalling, cell differentiation and cell proliferation pathways was modulated by C. vulgaris during replicative senescence of human diploid fibroblasts.

Indexed as

AntioxidantsCatalaseCell DifferentiationCell ProliferationCells, CulturedCellular SenescenceChlorella vulgarisDiploidyDNA DamageFibroblastsGene ExpressionGenes, p53HumansMaleMitogen-Activated Protein Kinase 14Molecular ChaperonesAntioxidantsCatalaseCAT protein, humanCCS protein, humanMitogen-Activated Protein Kinase 14Molecular ChaperonesSOD1 protein, humanSuperoxide DismutaseSuperoxide Dismutase-1Superoxide Dismutase 2Chlorella vulgarisFibroblastsReplicative senescenceSenescence-associated genes

Identifiers

PMID31642042
OpenAlexW2982161436

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.