Evidence map›Paper›PMID 31641153›Full record

ArticleScientific reports2019

Identification of microRNAs and relative target genes in Moringa oleifera leaf and callus.

Stefano Pirrò, Ivana Matic, Arianna Guidi, Letizia Zanella, Angelo Gismondi, Rosella Cicconi, Roberta Bernardini, Vittorio Colizzi, Antonella Canini, Maurizio Mattei and 1 more

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Cold Stress Responses and Adaptation Mechanisms inPlants (Basel, Switzerland) · 2026
    Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. The antioxidant properties and microbial load ofSaudi journal of biological sciences · 2022
    Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Molecular and Hormonal Regulation of Leaf Morphogenesis in Arabidopsis.International journal of molecular sciences · 2020
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Stefano PirròMir-Nat s.r.l., Rome, 00133, Italy.ORCID http://orcid.org/0000-0002-8601-722X
Ivana MaticMir-Nat s.r.l., Rome, 00133, Italy.ORCID http://orcid.org/0000-0001-5671-3016
Arianna GuidiCIMETA, University of Rome Tor Vergata, Rome, Italy.
Letizia ZanellaDepartment of Biology, University of Rome Tor Vergata, Rome, Italy.
Angelo GismondiDepartment of Biology, University of Rome Tor Vergata, Rome, Italy.
Rosella CicconiCIMETA, University of Rome Tor Vergata, Rome, Italy.
Roberta BernardiniCIMETA, University of Rome Tor Vergata, Rome, Italy.
Vittorio ColizziMir-Nat s.r.l., Rome, 00133, Italy.
Antonella CaniniDepartment of Biology, University of Rome Tor Vergata, Rome, Italy.ORCID http://orcid.org/0000-0003-1132-8899
Maurizio MatteiCIMETA, University of Rome Tor Vergata, Rome, Italy.
Andrea GalganiMir-Nat s.r.l., Rome, 00133, Italy. galgani@scienze.uniroma2.it.
University of Rome Tor Vergata · ITQueen Mary University of London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs, a class of small, non-coding RNAs, play important roles in plant growth, development and stress response by negatively regulating gene expression. Moringa oleifera Lam. plant has many medical and nutritional uses; however, little attention has been dedicated to its potential for the bio production of active compounds. In this study, 431 conserved and 392 novel microRNA families were identified and 9 novel small RNA libraries constructed from leaf, and cold stress treated callus, using high-throughput sequencing technology. Based on the M. oleifera genome, the microRNA repertoire of the seed was re-evaluated. qRT-PCR analysis confirmed the expression pattern of 11 conserved microRNAs in all groups. MicroRNA159 was found to be the most abundant conserved microRNA in leaf and callus, while microRNA393 was most abundantly expressed in the seed. The majority of predicted microRNA target genes were transcriptional factors involved in plant reproduction, growth/development and abiotic/biotic stress response. In conclusion, this is the first comprehensive analysis of microRNAs in M. oleifera leaf and callus which represents an important addition to the existing M. oleifera seed microRNA database and allows for possible exploitation of plant microRNAs induced with abiotic stress, as a tool for bio-enrichment with pharmacologically important phytochemicals.

Indexed as

Gene Expression Regulation, PlantGenes, PlantCold TemperatureGene OntologyMicroRNAsMoringa oleiferaPlant LeavesReproducibility of ResultsRNA, PlantMicroRNAsRNA, Plant

Identifiers

PMID31641153
PMCPMC6805943
OpenAlexW2982140705

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.