Evidence map›Paper›PMID 31640672›Full record

ArticleBMC veterinary research2019

Evaluation of Mycobacterium tuberculosis lipoarabinomannan antigen assay and rapid serology blood test for the diagnosis of bovine tuberculosis in Ethiopia.

Aboma Zewude, Temesgen Mohammed, Lemma Terfassa, W Garrett Hunt, Xueliang Pan, Joan Miquel Balada-Llasat, Wondwossen Gebreyes, Jordi B Torrelles, Shu-Hua Wang, Gobena Ameni

Open access · goldAbstract readEvaluation StudyValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. B Cell and Antibody Responses in Bovine Tuberculosis.Antibodies (Basel, Switzerland) · 2024
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Aboma ZewudeAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Temesgen MohammedAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Lemma TerfassaAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
W Garrett HuntNationwide Children's Hospital, Section of Infectious Diseases, College of Medicine, The Ohio State University, Columbus, OH, USA.
Xueliang PanCenter for Biostatistics, College of Medicine, The Ohio State University, Columbus, OH, USA.
Joan Miquel Balada-LlasatDepartment of Pathology, College of Medicine, The Ohio State University, Columbus, OH, USA.
Wondwossen GebreyesGlobal One Health initiative (GOHi), The Ohio State University, Columbus, OH, USA.
Jordi B TorrellesDepartment of Microbial Infection and Immunity, College of Medicine, The Ohio State University, Columbus, OH, USA.
Shu-Hua WangGlobal One Health initiative (GOHi), The Ohio State University, Columbus, OH, USA. Shu-Hua.Wang@osumc.edu.ORCID http://orcid.org/0000-0001-8006-2401
Gobena AmeniAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Addis Ababa University · ETThe Ohio State University · USNationwide Children's Hospital · USTexas Biomedical Research Institute · US

Funding

The Ohio State University Center for clinical and Translational ScienceUL1TR001070 · NCATS · OHIO STATE UNIVERSITY · PI JACKSON, REBECCA D · 2013 to 2017
$22.6M
Ethiopia-Emory TB Research Training ProgramD43TW009127 · FIC · EMORY UNIVERSITY · PI HENRY M BLUMBERG, Kidist Bobosha · 2013 to 2026
$3.4M
Center for Clinical and Translational Science, Ohio State University UL1TR001070FIC NIH HHS D43 TW009127NCATS NIH HHS UL1 TR001070
6 · The paper itself

Abstract

backgroundBovine tuberculosis (bTB) is prevalent in dairy cattle in Ethiopia. Currently used diagnostic tools such as the single intradermal comparative tuberculin test (SICTT) are time consuming and labor intensive. A rapid, easy-to-use and cost-effective diagnostic test would greatly contribute to the control of bTB in developing countries like Ethiopia. In the present study, two point-of-care diagnostic tests were evaluated for the detection of bTB: LIONEX® Animal TB Rapid test, a membrane-based test for the detection of antibodies to Mycobacterium bovis in blood and ALERE® Determine TB Lipoarabinomannan (LAM) Ag, an immunoassay for the detection of lipoarabinomannan (LAM) antigen (Ag) of mycobacteria in urine. A combination of the SICTT and gamma interferon (IFN-γ) test was used as the gold standard for the validation of these point-of-care tests, as it was not feasible to slaughter the study animals to carry out the historical gold standard of mycobacterial culture. A total of 175 heads of cattle having three different bTB infection categories (positive SICTT, negative SICTT, and unknown SICTT status) were used for this study.

resultThe sensitivity and specificity of TB LAM Ag were 72.2% (95% CI = 62.2, 80.4) and 98.8% (95% CI = 93.6, 99.7), respectively, while the sensitivity and specificity of the LIONEX Animal TB rapid test assay were 54% (95% CI = 44.1 64.3) and 98.8% (95% CI = 93.6, 99.7) respectively. The agreement between TB LAM Ag and SICTT was higher (κ = 0.85; 95% CI = 0.65-0.94) than between TB LAM Ag and IFN-γ (κ = 0.67; 95% CI = 0.52-0.81). The agreement between LIONEX Animals TB Rapid blood test and SICTT was substantial, (κ = 0.63; 95% CI = 0.49-0.77) while the agreement between LIONEX Animal TB rapid blood test and IFN-γ test was moderate (κ = 0.53; 95% CI = 0.40-0.67). Analysis of receiver operating curve (ROC) indicated that the area under the ROC curve (AUC) for TB LAM Ag was 0.85 (95% CI = 0.79-0.91) while it was 0.76 (95% CI; =0.69-0.83) for LIONEX Animal TB rapid test assay.

conclusionThis study showed that TB LAM Ag had a better diagnostic performance and could potentially be used as ancillary either to SICTT or IFN-γ test for diagnosis of bTB.

Indexed as

AnimalsAntibodies, BacterialCattleEthiopiaImmunoassayInterferon-gammaLipopolysaccharidesMycobacterium tuberculosisSensitivity and SpecificityTuberculin TestTuberculosis, BovineAntibodies, BacterialInterferon-gammalipoarabinomannanLipopolysaccharidesBovine tuberculosisDiagnostic performanceEthiopiaLIONEX animal TB rapidTB LAM antigen

Identifiers

PMID31640672
PMCPMC6805383
OpenAlexW2981902132

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.