ArticleBMC biotechnology2019
Standardized mounting method of (zebrafish) embryos using a 3D-printed stamp for high-content, semi-automated confocal imaging.
Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Laminin α5 and integrins α3 and α6 coordinately regulate collective cell migration in vivo.Development (Cambridge, England) · 2026Article
- Two distinct modes of Vgll4-mediated Tead regulation control organ size in zebrafish.Communications biology · 2026Article
- Customizable FDM-based zebrafish larva mold for live imaging.Biology open · 2026Article
- Real-time characterisation of microbe-induced inflammation using a novel zebrafish larval corneal injury and infection model.Communications biology · 2026Article
- Zebrafish (Biology · 2025Article
- Molecular and Functional Divergence of Zebrafish Sox Paralogs Controlling Endoderm Formation and Left-Right Patterning.Genome biology and evolution · 2025Article
- Acoustic Tweezers for Microscopy of Living Organisms.IEEE transactions on bio-medical engineering · 2025Article
- Custom 3D-Printed Molds for Zebrafish Imaging and Cardiac Development.Journal of visualized experiments : JoVE · 2025Article
- A method for creating custom 3D-printed molds to facilitate zebrafish imaging studies, including of cardiac development.bioRxiv : the preprint server for biology · 2025Article
- Phased ERK responsiveness and developmental robustness regulate teleost skin morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Agarose stamped method: a simple and customizable immobilization technique for zebrafish larvae.Frontiers in behavioral neuroscience · 2025Article
- Design and fabrication of customized brain slice matrices using CAD and 3D printing technology.PloS one · 2025Article
- Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization.Bioengineering (Basel, Switzerland) · 2024Article
- A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system.PloS one · 2024Article
- A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system.bioRxiv : the preprint server for biology · 2023Article
- Modeling Human Cardiac Arrhythmias: Insights from Zebrafish.Journal of cardiovascular development and disease · 2022Review
- The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics.Scientific reports · 2021Article
- Tools to Image Germplasm Dynamics During Early Zebrafish Development.Frontiers in cell and developmental biology · 2021Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDevelopmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the zebrafish Danio rerio in which developing tissues and organs can be easily observed and imaged using fluorescent proteins have become very popular. One limiting factor however is the acquisition of a sufficient amount of data, in standardized and reproducible conditions, to allow robust quantitative analysis. One way to improve this is by developing mounting methods to increase the number of embryos that can be imaged simultaneously in near-to-identical orientation.
resultsHere we present an improved mounting method allowing semi-automated and high-content imaging of zebrafish embryos. It is based on a 3D-printed stamp which is used to create a 2D coordinate system of multiple μ-wells in an agarose cast. Each μ-well models a negative of the average zebrafish embryo morphology between 22 and 96 h-post-fertilization. Due to this standardized and reproducible arrangement, it is possible to define a custom well plate in the respective imaging software that allows for a semi-automated imaging process. Furthermore, the improvement in Z-orientation significantly reduces post-processing and improves comparability of volumetric data while reducing light exposure and thus photo-bleaching and photo-toxicity, and improving signal-to-noise ratio (SNR).
conclusionsWe present here a new method that allows to standardize and improve mounting and imaging of embryos. The 3D-printed stamp creates a 2D coordinate system of μ-wells in an agarose cast thus standardizing specimen mounting and allowing high-content imaging of up to 44 live or mounted zebrafish embryos simultaneously in a semi-automated, well-plate like manner on inverted confocal microscopes. In summary, image data quality and acquisition efficiency (amount of data per time) are significantly improved. The latter might also be crucial when using the services of a microscopy facility.
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