Evidence map›Paper›PMID 31640560›Full record

ArticleBMC genomics2019

Transcriptomic analysis of Macrobrachium rosenbergii (giant fresh water prawn) post-larvae in response to M. rosenbergii nodavirus (MrNV) infection: de novo assembly and functional annotation.

Phongthana Pasookhush, Charles Hindmarch, Paisarn Sithigorngul, Siwaporn Longyant, William G Bendena, Parin Chaivisuthangkura

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 39 citations in OpenAlex.

  1. Review
  2. Animals : an open access journal from MDPI · 2025
    Review
  3. Article
  4. Review
  5. Growth and Welfare Status of Giant Freshwater Prawn (Animals : an open access journal from MDPI · 2023
    Article
  6. Review
  7. Article
  8. Molecular Cytogenetic Analysis in Freshwater Prawns of the GenusInternational journal of molecular sciences · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Phongthana PasookhushDepartment of Biology, Srinakharinwirot University, Sukhumvit 23, Bangkok, 10110, Thailand.
Charles HindmarchQCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Kingston, ON, K7L 3N6, Canada.
Paisarn SithigorngulDepartment of Biology, Srinakharinwirot University, Sukhumvit 23, Bangkok, 10110, Thailand.
Siwaporn LongyantDepartment of Biology, Srinakharinwirot University, Sukhumvit 23, Bangkok, 10110, Thailand.
William G BendenaDepartment of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada.
Parin ChaivisuthangkuraDepartment of Biology, Srinakharinwirot University, Sukhumvit 23, Bangkok, 10110, Thailand. parin@g.swu.ac.th.ORCID http://orcid.org/0000-0002-8326-222X
Srinakharinwirot University · THQueen's University · CA

Funding

CATHEPSIN B-LIKE CYSTEINE PROTEINASES AND TUMOR INVASIONR01CA036481 · NCI · WAYNE STATE UNIVERSITY · PI SLOANE, BONNIE F · 1985 to 2005
$1.7M
Natural Sciences and Engineering Research Council of Canada Discovery Grant RGPIN36481-08Strategic Wisdom and Research Institute, Srinakharinwirot University Grant 520/2561
6 · The paper itself

Abstract

backgroundMacrobrachium rosenbergii, is one of a major freshwater prawn species cultured in Southeast Asia. White tail disease (WTD), caused by Macrobrachium rosenbergii nodavirus (MrNV), is a serious problem in farm cultivation and is responsible for up to 100% mortality in the post larvae stage. Molecular data on how M. rosenbergii post-larvae launches an immune response to an infection with MrNV is not currently available. We therefore compared the whole transcriptomic sequence of M. rosenbergii post-larvae before and after MrNV infection.

resultsTranscriptome for M. rosenbergii post-larvae demonstrated high completeness (BUSCO Complete: 83.4%, fragmentation: 13%, missing:3.3%, duplication:16.2%; highest ExN50 value: 94%). The assembled transcriptome consists of 96,362 unigenes with N

conclusionsThis study reported a highly complete transcriptome from the post-larvae stage of giant river prawn, M. rosenbergii. Differential abundant transcripts during MrNV infection were identified and validated by qPCR, many of these differentially abundant transcripts as key players in antiviral immunity. These include known members of the innate immune response with the largest expression change occurring in the M. rosenbergii post-larvae after MrNV infection such as antiviral protein, C-type lectin, prophenol oxidase, caspase, ADP ribosylation factors, and dicer.

Indexed as

AnimalsAquacultureFresh WaterGene Expression ProfilingGene OntologyImmunityMolecular Sequence AnnotationNodaviridaePalaemonidaeRNA Virus InfectionsTranscriptomeCrustacean immunityde novo transcriptome assemblyDifferential expression analysisMacrobrachium rosenbergiiM. rosenbergii nodavirusRNAseq

Identifiers

PMID31640560
PMCPMC6805343
OpenAlexW2981572045

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.