Evidence map›Paper›PMID 31639592›Full record

ArticleWater research2020

Community members in activated sludge as determined by molecular probe technology.

Weihong Xu, Veronica R Brand, Sundari Suresh, Michael A Jensen, Ronald W Davis, Craig S Criddle, Robert P St Onge, Richard W Hyman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Water research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Weihong XuStanford Genome Technology Center, Palo Alto, CA, 94304, USA; Precision Medicine Institute, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Veronica R BrandDepartment of Civil and Environmental Engineering, Stanford University, Stanford, CA, 94305, USA.
Sundari SureshStanford Genome Technology Center, Palo Alto, CA, 94304, USA.
Michael A JensenStanford Genome Technology Center, Palo Alto, CA, 94304, USA.
Ronald W DavisStanford Genome Technology Center, Palo Alto, CA, 94304, USA; Department of Biochemistry, Stanford University Medical College, Stanford, CA, 94305, USA; Department of Genetics, Stanford University Medical College, Stanford, CA, 94305, USA.
Craig S CriddleDepartment of Civil and Environmental Engineering, Stanford University, Stanford, CA, 94305, USA.
Robert P St OngeStanford Genome Technology Center, Palo Alto, CA, 94304, USA; Department of Biochemistry, Stanford University Medical College, Stanford, CA, 94305, USA.
Richard W HymanStanford Genome Technology Center, Palo Alto, CA, 94304, USA; Department of Biochemistry, Stanford University Medical College, Stanford, CA, 94305, USA. Electronic address: rhyman@stanford.edu.

Funding

WHOLE YEAST GENOME TRANSCRIPTIONAL AND FUNCTIONAL GENE MAPS--A FEASIBILITY STUDYP01HG000205 · NHGRI · STANFORD UNIVERSITY · PI HOWE, ROGER THOMAS, JI, HANLEE P · 1990 to 2016
$120.9M
A NEW ES CELL DELETION RESOURCE AND WILLIAMS SYNDROMEF32HG000205 · NHGRI · JACKSON LABORATORY · PI GOODWIN, NEAL C · 1999 to 1999
–
NHGRI NIH HHS F32 HG000205NHGRI NIH HHS P01 HG000205
6 · The paper itself

Abstract

The use of molecular probe technology is demonstrated for routine identification and tracking of cultured and uncultured microorganisms in an activated sludge bioreactor treating domestic wastewater. A key advantage of molecular probe technology is that it can interrogate hundreds of microbial species of interest in a single measurement. In environmental niches where a single genus (such as Competibacteraceae) dominates, it can be difficult and expensive to identify microorganisms that are present at low relative abundance. With molecular probe technology, it is straightforward. Members of the Competibacteraceae family, none of which have been grown in pure culture, are abundant in an activated sludge system in the San Francisco Bay Area, California, USA. Molecular probe ensembles with and without Competibacteraceae probes were constructed. Whereas the probe ensemble with Competibacteraceae probes identified a total of ten bacteria, the molecular probe ensemble without Competibacteraceae probes identified 29 bacteria, including many at low relative abundance and including some species of public health significance.

Indexed as

Molecular ProbesSewageBioreactorsRNA, Ribosomal, 16SSan FranciscoWastewaterMolecular ProbesRNA, Ribosomal, 16SSewageWastewaterActivated sludgeBacteriaMolecular probes

Identifiers

PMID31639592
PMCPMC6873225

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.