Evidence map›Paper›PMID 31639222›Full record

ArticleFEBS letters2020

Fis protein forms DNA topological barriers to confine transcription-coupled DNA supercoiling in Escherichia coli.

Samantha Dages, Xiaoduo Zhi, Fenfei Leng

Open access · bronzeAbstract read
In one paragraph

Article in FEBS letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. An Engineered Variant ofACS synthetic biology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Samantha DagesBiomolecular Sciences Institute and Department of Chemistry & Biochemistry, Florida International University, Miami, FL, USA.
Xiaoduo ZhiBiomolecular Sciences Institute and Department of Chemistry & Biochemistry, Florida International University, Miami, FL, USA.
Fenfei LengBiomolecular Sciences Institute and Department of Chemistry & Biochemistry, Florida International University, Miami, FL, USA.ORCID 0000-0002-9024-1216
Florida International University · US

Funding

Targeting transcription-coupled DNA supercoiling for discovering antibiotics against bacterial DNA gyraseR21AI125973 · NIAID · FLORIDA INTERNATIONAL UNIVERSITY · PI LENG, FENFEI · 2017 to 2018
$478k
Transcription-Coupled DNA SupercoilingR15GM109254 · NIGMS · FLORIDA INTERNATIONAL UNIVERSITY · PI LENG, FENFEI · 2014 to 2014
$317k
NIAID NIH HHS R21 AI125973NIGMS NIH HHS R15 GM109254NIH HHS 1R21AI125973-01A1
6 · The paper itself

Abstract

Previously, we demonstrated that transcription-coupled DNA supercoiling (TCDS) potently activated or inhibited nearby promoters in Escherichia coli even in the presence of all four DNA topoisomerases, suggesting that DNA topoisomerases are not the only factors regulating TCDS. A different mechanism exists to confine this localized DNA supercoiling. Using an in vivo system containing the TCDS-activated leu-500 promoter (P

Indexed as

Promoter Regions, GeneticDNA, BacterialDNA, SuperhelicalEscherichia coliEscherichia coli ProteinsFactor For Inversion Stimulation ProteinGene DeletionGene Expression Regulation, BacterialNucleic Acid ConformationTranscription FactorsTranscription, GeneticDNA, BacterialDNA, SuperhelicalEscherichia coli ProteinsFactor For Inversion Stimulation ProteinFis protein, E coliIbaG protein, E coliTranscription FactorsDNA supercoilingDNA topological barriersEscherichia coliFis protein

Identifiers

PMID31639222
PMCPMC10857741
OpenAlexW2981659952

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.