ArticleBMC plant biology2019
The interplay between miR156/SPL13 and DFR/WD40-1 regulate drought tolerance in alfalfa.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 140 citations in OpenAlex.
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- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
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- Exploring the drought-responsive miRNAs and their corresponding target genes in chickpea root tissue.Plant molecular biology · 2025Article
- Phenylpropanoids metabolism: recent insight into stress tolerance and plant development cues.Frontiers in plant science · 2025Review
- Understanding abiotic stress in alfalfa: physiological and molecular perspectives on salinity, drought, and heavy metal toxicity.Frontiers in plant science · 2025Review
- TheFrontiers in plant science · 2025Article
- Transcriptional Profiling Analysis Providing Insights into the Harsh Environments Tolerance Mechanisms ofInternational journal of molecular sciences · 2024Article
- Screening of mtr-miR156a from exosomes of dairy cow blood to milk and its regulatory effect on milk protein synthesis in BMECs.BMC genomics · 2024Article
- Omics approaches to understand the MADS-box gene family in common bean (Phaseolus vulgaris L.) against drought stress.Protoplasma · 2024Article
- Genome-wide identification and analysis of WD40 proteins reveal that NtTTG1 enhances drought tolerance in tobacco (Nicotiana tabacum).BMC genomics · 2024Article
- Whole-Transcriptome Sequencing Reveals the Global Molecular Responses and NAC Transcription Factors Involved in Drought Stress inAntioxidants (Basel, Switzerland) · 2024Article
- Identification and Validation of the miR156 Family Involved in Drought Responses and Tolerance in Tea Plants (Plants (Basel, Switzerland) · 2024Article
- Research on drought stress inFrontiers in plant science · 2024Review
- Pepper SBP-box transcription factor,Frontiers in plant science · 2024Article
- Comprehensive analysis of transcriptome and metabolome provides insights into the stress response mechanisms of apple fruit to postharvest impact damage.Food chemistry. Molecular sciences · 2023Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDeveloping Medicago sativa L. (alfalfa) cultivars tolerant to drought is critical for the crop's sustainable production. miR156 regulates various plant biological functions by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors.
resultsTo understand the mechanism of miR156-modulated drought stress tolerance in alfalfa we used genotypes with altered expression levels of miR156, miR156-regulated SPL13, and DIHYDROFLAVONOL-4-REDUCTASE (DFR) regulating WD40-1. Previously we reported the involvement of miR156 in drought tolerance, but the mechanism and downstream genes involved in this process were not fully studied. Here we illustrate the interplay between miR156/SPL13 and WD40-1/DFR to regulate drought stress by coordinating gene expression with metabolite and physiological strategies. Low to moderate levels of miR156 overexpression suppressed SPL13 and increased WD40-1 to fine-tune DFR expression for enhanced anthocyanin biosynthesis. This, in combination with other accumulated stress mitigating metabolites and physiological responses, improved drought tolerance. We also demonstrated that SPL13 binds in vivo to the DFR promoter to regulate its expression.
conclusionsTaken together, our results reveal that moderate relative miR156 transcript levels are sufficient to enhance drought resilience in alfalfa by silencing SPL13 and increasing WD40-1 expression, whereas higher miR156 overexpression results in drought susceptibility.
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