ArticleMicrobiologyOpen2020
Method for the facile transformation of marine purple photosynthetic bacteria using chemically competent cells.
Article in MicrobiologyOpen, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- The Biology of Natural Polymers Accelerates and Expands the Science of Biomacromolecules: A Focus on Structural Proteins.Biomacromolecules · 2025Review
- Low-salinity medium for large-scale biomass production of the marine purple photosynthetic bacterium Rhodovulum sulfidophilum.PloS one · 2025Article
- Outer Membrane Vesicles Derived fromInternational journal of molecular sciences · 2021Article
- An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Peptide-Mediated Gene Transfer into Marine Purple Photosynthetic Bacteria.International journal of molecular sciences · 2020Article
- Method for the facile transformation of marine purple photosynthetic bacteria using chemically competent cells.MicrobiologyOpen · 2020Article
- Marine Purple Photosynthetic Bacteria as Sustainable Microbial Production Hosts.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine purple photosynthetic bacteria are ideal organisms for the production of useful materials at reduced costs and contributing to a sustainable society because they can utilize sunlight, seawater, and components of air, including carbon dioxide and nitrogen gases, for their growth. However, conjugation is the only applicable method for the transformation of marine purple photosynthetic bacteria so far. Here, we examined a calcium chloride-mediated method for the transformation of marine purple photosynthetic bacteria. Plasmid DNAs containing the kanamycin resistance gene were successfully transferred into chemically competent cells of two strains of marine purple photosynthetic bacteria (Rhodovulum sulfidophilum and Roseospira marina). Heat shock treatment increased the transformation efficiency in R. sulfidophilum, whereas the addition of cell-penetrating peptide did not improve it. We also found that prolonged incubation in agar plates containing kanamycin led to spontaneous mutation of the 16S rRNA, resulting in kanamycin resistance in R. marina. Thus, we developed an efficient and facile transformation method using chemically competent cells of marine purple photosynthetic bacteria with calcium chloride.
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