Evidence map›Paper›PMID 31638242›Full record

ArticleOncology reports2019

miRNA‑183‑5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1.

Jun Lin, Juan Shen, Hao Yue, Zhongwei Cao

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
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  5. Sex differences in human pre-gastrulation embryos.Science China. Life sciences · 2025
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  11. Research Advances in the Role of the Tropomyosin Family in Cancer.International journal of molecular sciences · 2023
    Review
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  15. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jun LinDepartment of Pathology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China.
Juan ShenDepartment of Pathology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China.
Hao YueDepartment of Pathology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China.
Zhongwei CaoDepartment of Gastroenterology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China.
Shanghai First People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNA‑183 (miR‑183) is a small, non‑coding RNA that is involved in post‑transcriptional processes, is upregulated in gastric cancer and acts as an oncogene in cancer migration. Although fragmentary reports have demonstrated the importance of miR‑183 in gastric cancer, its biofunctions and regulatory effects are still unknown. In the present study, the gene and protein expression levels were determined by reverse transcription‑quantitative PCR and western blot analysis. The connection between miR‑183‑5p.1 and tropomyosin 1 (TPM1) was tested through luciferase reporter experiments. Cell viability, apoptosis and related proteins were detected by MTT assay, flow cytometry, immunofluorescence and western blotting, respectively. The migration and invasion of AGS cells modulated by miR‑183‑5p.1 were analyzed by Transwell assay. TPM1 expression was found to be decreased in gastric cancer tissues and cell lines when compared with normal and adjacent tissues and gastric epithelial cells, and was regulated by miR‑183‑5p.1 targeting TPM1. miR‑183‑5p.1 overexpression facilitated the growth and suppressed the death of AGS cells through Bcl‑2 and P53 proteins. In addition, miR‑183‑5p.1 restricted TPM1, TPM2 and TPM3 protein expression in AGS cells. The excessive levels of miR‑183‑5p.1 promoted the migration and invasion of AGS cells, and inhibited the apoptosis of AGS cells. However, the knockdown of miR‑183‑5p.1 induced the opposite in AGS cells. In conclusion, miR‑183‑5p.1 promotes cell proliferation, migration and invasion by downregulating TPM1 and deactivating the Bcl‑2/P53 signaling pathways in gastric cancer, indicating that miR‑183‑5p.1 and TPM1 may be potential targets for the diagnosis or therapy of gastric cancer in the future.

Indexed as

Cell MovementGene Expression Regulation, NeoplasticAdultAgedApoptosisBiomarkers, TumorCell ProliferationFemaleFollow-Up StudiesHumansMaleMicroRNAsMiddle AgedNeoplasm InvasivenessPrognosisStomach NeoplasmsBiomarkers, TumorMicroRNAsMIRN183 microRNA, humanTPM1 protein, humanTropomyosin

Identifiers

PMID31638242
PMCPMC6859460
OpenAlexW2978597592

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.