ArticleMolecular medicine reports2019
miR‑222 induces apoptosis in human intervertebral disc nucleus pulposus cells by targeting Bcl‑2.
Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 13 citations in OpenAlex.
- Programmed cell death in degenerative skeletal diseases: molecular crosstalk and combinatorial therapeutic strategies.Frontiers in cell and developmental biology · 2026Review
- Apoptotic Pathway in Intervertebral Disc Degeneration: From Molecular Pathways to Clinical Interventions.Diagnostics (Basel, Switzerland) · 2025Review
- MicroRNAs regulating apoptosis and delivery strategies for treating intervertebral disc degeneration.Frontiers in cell and developmental biology · 2025Review
- Mechanobiology of MicroRNAs in Intervertebral Disk Degeneration.Journal of spine research and surgery · 2023Article
- Role of Caspase Family in Intervertebral Disc Degeneration and Its Therapeutic Prospects.Biomolecules · 2022Review
- Treatment of Intervertebral Disc Degeneration.Orthopaedic surgery · 2022Review
- Panax notoginseng saponin reducesAnnals of translational medicine · 2022Article
- MiR-206 improves intervertebral disk degeneration by targeting GJA1.Journal of orthopaedic surgery and research · 2022Article
- The Mechanism and Function of miRNA in Intervertebral Disc Degeneration.Orthopaedic surgery · 2022Review
- M1 macrophage-derived exosomes transfer miR-222 to induce bone marrow mesenchymal stem cell apoptosis.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting theFrontiers in genetics · 2020Article
- Knockdown of hsa_circ_0059955 Induces Apoptosis and Cell Cycle Arrest in Nucleus Pulposus Cells via InhibitingDrug design, development and therapy · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intervertebral disc degeneration (IDD) is characterized by abnormal induction of apoptosis in intervertebral disc nucleus pulposus (NP) cells. Previous studies indicated that miR‑222 was upregulated in patients with rheumatoid arthritis. However, the effects of miR‑222 in IDD remain unclear. The present study aimed to demonstrate the role of miR‑222 in NP cells. The levels of miR‑222 in patients with IDD were measured by reverse transcription‑quantitative PCR. Cell Counting Kit‑8 and western blotting assays were used to detect cell proliferation and apoptosis‑associated protein levels, respectively. In addition, luciferase reporter assays were performed to validate the predicted target genes of miR‑222. miR‑222 was significantly upregulated in patients with IDD. Overexpression of miR‑222 inhibited cell proliferation and induced cell apoptosis. Moreover, overexpression of miR‑222 resulted in an upregulation in the levels of Bax and cleaved caspase 3, and a downregulation in the levels of Bcl‑2 in NP cells. The luciferase reporter assays demonstrated that Bcl‑2 is a target of miR‑222. Furthermore, overexpression of miR‑222 increased the levels of cytochrome c, apoptotic protease activating factor‑1 and cleaved caspase 9 in NP cells. Conversely, downregulation of miR‑222 could promote the proliferation of NP cells. The present data demonstrated that miR‑222 induced apoptosis in NP cells by directly targeting Bcl‑2. Therefore, miR‑222 may act as a potential therapeutic target for the treatment of IDD.
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