ArticlePlant biotechnology journal2020
Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
110 citing papers in PubMed, 238 citations in OpenAlex.
- Review
- Integrated multi-omics profiling reveals dynamic regulation of light-induced chloroplast biogenesis in Brassica napus seedlings.Nucleic acids research · 2026Article
- Review
- The BnTFL1-BnJAM3-BnSWEETs Module Orchestrates Seed Storage Reserve Accumulation in Brassica napus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- T2T reference genome assembly provides insights into anthocyanin accumulation in broccoli.Horticulture research · 2026Article
- Identification of candidate genes regulating seed oil and protein content in Brassica napus via integrated genome-wide association and transcriptome analysis.BMC plant biology · 2026Article
- BnaCIPK9 homoeologs mediate the dosage-dependent regulation of seed oil in allotetraploid Brassica napus L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- A pennycress transparent testa 8 knockout mutant has drastic changes in seed coat anatomy and chemical compositions.Journal of experimental botany · 2026Article
- Dissecting the genetic architecture of seed-related traits in Brassica napus by integrating multi-omics analysis and VIS-NIR hyperspectral imaging.Genome biology · 2026Article
- Exploring metabolite diversity in global rapeseed germplasm: insights into ecotypes, geographical influences, and correlations with diabetes.BMC plant biology · 2025Article
- Transcription Factor BnaC04.MYB89 Negatively Regulates Seed Fatty Acid Biosynthesis inPlants (Basel, Switzerland) · 2025Article
- ANTHOCYANIDIN REDUCTASE promotes physical dormancy in Medicago truncatula seeds.Plant physiology · 2025Article
- Plant oil biosynthesis and genetic improvement: progress, challenges, and opportunities.Plant physiology · 2025Review
- Mutations of two AhTT8 genes reduce the accumulation of anthocyanins and confer the white testa of peanut (Arachis hypogaea L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Efficient and versatile rapeseed transformation for new breeding technologies.The Plant journal : for cell and molecular biology · 2025Article
- Complexity Meets Risk-The Next Generation of Genome-Edited Plants Challenges Established Concepts for Environmental Risk Assessment in the EU.Plants (Basel, Switzerland) · 2025Review
- Review
- Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement.Stress biology · 2025Review
- Article
- Untargeted Metabolomics Analysis Reveals Differential Accumulation of Flavonoids Between Yellow-Seeded and Black-Seeded Rapeseed Varieties.Plants (Basel, Switzerland) · 2025Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yellow seed is a desirable trait with great potential for improving seed quality in Brassica crops. Unfortunately, no natural or induced yellow seed germplasms have been found in Brassica napus, an important oil crop, which likely reflects its genome complexity and the difficulty of the simultaneous random mutagenesis of multiple gene copies with functional redundancy. Here, we demonstrate the first application of CRISPR/Cas9 for creating yellow-seeded mutants in rapeseed. The targeted mutations of the BnTT8 gene were stably transmitted to successive generations, and a range of homozygous mutants with loss-of-function alleles of the target genes were obtained for phenotyping. The yellow-seeded phenotype could be recovered only in targeted mutants of both BnTT8 functional copies, indicating that the redundant roles of BnA09.TT8 and BnC09.TT8b are vital for seed colour. The BnTT8 double mutants produced seeds with elevated seed oil and protein content and altered fatty acid (FA) composition without any serious defects in the yield-related traits, making it a valuable resource for rapeseed breeding programmes. Chemical staining and histological analysis showed that the targeted mutations of BnTT8 completely blocked the proanthocyanidin (PA)-specific deposition in the seed coat. Further, transcriptomic profiling revealed that the targeted mutations of BnTT8 resulted in the broad suppression of phenylpropanoid/flavonoid biosynthesis genes, which indicated a much more complex molecular mechanism underlying seed colour formation in rapeseed than in Arabidopsis and other Brassica species. In addition, gene expression analysis revealed the possible mechanism through which BnTT8 altered the oil content and fatty acid composition in seeds.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.