Evidence map›Paper›PMID 31637135›Full record

ArticlePeerJ2019

Tissue-specific evaluation of suitable reference genes for RT-qPCR in the pond snail,

Alexander P Young, Carmen F Landry, Daniel J Jackson, Russell C Wyeth

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 17 citations in OpenAlex.

  1. Altitude-Related Adaptation in Freshwater Snails (Animals : an open access journal from MDPI · 2026
    Article
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  6. JAK1/2 Regulates Synergy Between Interferon Gamma and Lipopolysaccharides in Microglia.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Alexander P YoungDepartment of Biology, St. Francis Xavier University, Antigonish, NS, Canada.ORCID 0000-0003-3395-2377
Carmen F LandryDepartment of Biology, St. Francis Xavier University, Antigonish, NS, Canada.
Daniel J JacksonDepartment of Geobiology, Georg-August Universität Göttingen, Göttingen, Germany.ORCID 0000-0001-9045-381X
Russell C WyethDepartment of Biology, St. Francis Xavier University, Antigonish, NS, Canada.ORCID 0000-0003-0971-7588
St. Francis Xavier University · CAUniversity of Göttingen · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reverse transcription quantitative PCR (RT-qPCR) is a robust technique for the quantification and comparison of gene expression. To obtain reliable results with this method, one or more reference genes must be employed to normalize expression measurements among treatments or tissue samples. Candidate reference genes must be validated to ensure that they are stable prior to use in qPCR experiments. The pond snail (

Indexed as

Gastropod neurobiologyGene expressionMolluscNormalization

Identifiers

PMID31637135
PMCPMC6798871
OpenAlexW2963686947

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.