ArticleMicrosystems & nanoengineering2019
Rapid two-dimensional characterisation of proteins in solution.
Article in Microsystems & nanoengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Microfluidic diffusional sizing in bioanalysis and biosensing.Lab on a chip · 2026Review
- Enhancing cell characterization with microfluidics and AI: a comprehensive review of mechanical, electrical, and hybrid techniques.Biotechnology reports (Amsterdam, Netherlands) · 2025Review
- Single-molecule digital sizing of proteins in solution.Nature communications · 2024Article
- Microfluidics for multiscale studies of biomolecular condensates.Lab on a chip · 2022Review
- Microscale Diffusiophoresis of Proteins.The journal of physical chemistry. B · 2022Article
- Microfluidics - The State-of-the-Art Technology for Pharmaceutical Application.Advanced pharmaceutical bulletin · 2022Review
- Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device.Micromachines · 2022Article
- Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures.Biomedicines · 2022Review
- Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles.Biosensors · 2021Review
- Machine learning-aided protein identification from multidimensional signatures.Lab on a chip · 2021Article
- Fine-Tuning Electrokinetic Injections Considering Nonlinear Electrokinetic Effects in Insulator-Based Devices.Micromachines · 2021Article
- Microchip Free-Flow Electrophoresis for Bioanalysis, Sensing, and Purification.Methods in molecular biology (Clifton, N.J.)Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidic platforms provide an excellent basis for working with heterogeneous samples and separating biomolecular components at high throughput, with high recovery rates and by using only very small sample volumes. To date, several micron scale platforms with preparative capabilities have been demonstrated. Here we describe and demonstrate a microfluidic device that brings preparative and analytical operations together onto a single chip and thereby allows the acquisition of multidimensional information. We achieve this objective by using a free-flow electrophoretic separation approach that directs fractions of sample into an on-chip analysis unit, where the fractions are characterised through a microfluidic diffusional sizing process. This combined approach therefore allows simultaneously quantifying the sizes and the charges of components in heterogenous mixtures. We illustrate the power of the platform by describing the size distribution of a mixture comprising components which are close in size and cannot be identified as individual components using state-of-the-art solution sizing techniques on their own. Furthermore, we show that the platform can be used for two-dimensional fingerprinting of heterogeneous protein mixtures within tens of seconds, opening up a possibility to obtain multiparameter data on biomolecular systems on a minute timescale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.