ArticleProceedings of the National Academy of Sciences of the United States of America2019
Quantifying cellulose accessibility during enzyme-mediated deconstruction using 2 fluorescence-tagged carbohydrate-binding modules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Slowdown of Enzymatic Cellulose Conversion Emerges from Cellulase Mode of Action.ACS catalysis · 2026Article
- Metatranscriptomics uncovers diet-driven structural, ecological, and functional adaptations in the rumen microbiome linked to feed efficiency.ISME communications · 2026Article
- Are cellulases slow? Kinetic and thermodynamic limitations for enzymatic breakdown of cellulose.BBA advances · 2025Review
- Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce.Biotechnology for biofuels and bioproducts · 2023Article
- Detecting the orientation of newly-deposited crystalline cellulose with fluorescent CBM3.Cell surface (Amsterdam, Netherlands) · 2022Article
- Enzyme Synergy in Transient Clusters of Endo- and Exocellulase Enables a Multilayer Mode of Processive Depolymerization of Cellulose.ACS catalysis · 2022Article
- Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls.International journal of molecular sciences · 2022Article
- Synergic action of an inserted carbohydrate-binding module in a glycoside hydrolase family 5 endoglucanase.Acta crystallographica. Section D, Structural biology · 2022Article
- PACER: a novel 3D plant cell wall model for the analysis of non-catalytic and enzymatic responses.Biotechnology for biofuels and bioproducts · 2022Article
- Differences in Fecal Microbiome and Antimicrobial Resistance between Captive and Free-Range Sika Deer under the Same Exposure of Antibiotic Anthelmintics.Microbiology spectrum · 2021Article
- Addition of Various Cellulosic Components to Bacterial Nanocellulose: A Comparison of Surface Qualities and Crystalline Properties.Journal of microbiology and biotechnology · 2021Article
- Biocatalysis in the Recycling Landscape for Synthetic Polymers and Plastics towards Circular Textiles.ChemSusChem · 2021Review
- Towards sustainable production and utilization of plant-biomass-based nanomaterials: a review and analysis of recent developments.Biotechnology for biofuels · 2021Review
- The characteristics of insoluble softwood substrates affect fungal morphology, secretome composition, and hydrolytic efficiency of enzymes produced by Trichoderma reesei.Biotechnology for biofuels · 2021Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- Towards circular fashion - transforming pulp mills into hubs for textile recycling.RSC advances · 2021Article
- Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems.AoB PLANTS · 2020Article
- The use of fluorescent protein-tagged carbohydrate-binding modules to evaluate the influence of drying on cellulose accessibility and enzymatic hydrolysis.RSC advances · 2020Article
- Elucidation of Changes in Cellulose Ultrastructure and Accessibility in Hardwood Fractionation Processes with Carbohydrate Binding Modules.ACS sustainable chemistry & engineering · 2020Article
- Comparative Genomics ofGenes · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Two fluorescence-tagged carbohydrate-binding modules (CBMs), which specifically bind to crystalline (CBM2a-RRedX) and paracrystalline (CBM17-FITC) cellulose, were used to differentiate the supramolecular cellulose structures in bleached softwood Kraft fibers during enzyme-mediated hydrolysis. Differences in CBM adsorption were elucidated using confocal laser scanning microscopy (CLSM), and the structural changes occurring during enzyme-mediated deconstruction were quantified via the relative fluorescence intensities of the respective probes. It was apparent that a high degree of order (i.e., crystalline cellulose) occurred at the cellulose fiber surface, which was interspersed by zones of lower structural organization and increased cellulose accessibility. Quantitative image analysis, supported by
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.