Trial reportAnnals of epidemiology2019
High-throughput sequencing of pooled samples to determine community-level microbiome diversity.
Trial report in Annals of epidemiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Mass azithromycin distribution and antibiotic resistance in the gut and nasopharynx: a cluster-randomized trial.Nature medicine · 2026Trial
- Gut Microbiome Diversity and Antimicrobial Resistance After a Single Dose of Oral Azithromycin in Children: A Randomized Placebo-Controlled Trial.The American journal of tropical medicine and hygiene · 2024Trial
- Macrolide and Nonmacrolide Resistance with Mass Azithromycin Distribution.The New England journal of medicine · 2020Trial
- Regulatory Effects of Tannin Supplementation on Microbial Succession and Flavor Formation During Xiaoqu Light-Flavor Baijiu Fermentation.Foods (Basel, Switzerland) · 2026Article
- Microbiota variation inMicrobiology spectrum · 2026Article
- Exploring the functional microbiome of pigs within the porcine respiratory disease complex: viral-bacterial co-infections and virulence factor profiling.Microbiology spectrum · 2026Article
- Tagatose attenuates streptococcus mutans-induced dental caries by modulating oral microbiota and inflammatory responses in mice.BMC microbiology · 2026Article
- Metagenomic insights into oral microbiota dynamics in diabetic and non-diabetic periodontal disease: a pilot study.Frontiers in microbiology · 2026Article
- Mitochondrial diversity of Bwindi Impenetrable National Park Mountain Gorillas.Genome biology · 2025Article
- Age-Dependent Gut Microbiota Dynamics and Their Association with Male Life-History Traits in Drosophila melanogaster.Microbial ecology · 2025Article
- Role of Glial Trace Amine Associated Receptor 1 (TAAR1) and Microbiota in Schizophrenia.Neurochemical research · 2025Review
- The impact of pooling on the observed microbiome profile of preweaned piglet feces.FEMS microbiology ecology · 2025Article
- Wastewater Metavirome Diversity: Exploring Replicate Inconsistencies and Bioinformatic Tool Disparities.International journal of environmental research and public health · 2025Article
- Underscoring long-term host-microbiome interactions in a physiologically relevant gingival tissue model.NPJ biofilms and microbiomes · 2025Article
- Preliminary profile of the gut microbiota from amerindians in the Brazilian amazon experiencing a process of transition to urbanization.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Metagenomic analysis unravels novel taxonomic differences in the uterine microbiome between healthy mares and mares with endometritis.Veterinary medicine and science · 2024Article
- Gut Bacteriomes and Ecological Niche Divergence: An Example of Two Cryptic Gastropod Species.Biology · 2023Article
- Stevia as a natural additive on gut health and cecal microbiota in broilers.Veterinary and animal science · 2023Article
- Therapeutic Effects ofMicroorganisms · 2023Article
- A Scoping Review Evaluating the Current State of Gut Microbiota Research in Africa.Microorganisms · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
purposeCommunity-level interventions in cluster randomized controlled trials may alter the gut microbiome of individuals. The current method of estimating community diversities uses microbiome data obtained from multiple individual's specimens. Here we propose randomly pooling a number of microbiome samples from the same community into one sample before sequencing to estimate community-level microbiome diversity.
methodsWe design and analyze an experiment to compare community microbiome diversity (gamma-diversity) estimates derived from 16S rRNA gene sequencing of 1) individually sequenced specimens vs. 2) pooled specimens collected from a community. Pool sizes of 10, 20, and 40 are considered. We then compare the gamma-estimates using Pearson's correlation as well as using Bland and Altman agreement analysis for three established diversity indices including richness, Simpson's and Shannon's.
resultsThe gamma-diversity estimates are highly correlated, with most being statistically significant. All correlations between all three diversity estimates are significant in the 10-pooled data. Pools comprising 40 specimens are closest to the line of agreement, but all pooled samples and individual samples fall within the 95% limits of agreement.
conclusionsPooling microbiome samples before DNA amplification and metagenomics sequencing to estimate community-level diversity is a viable measure to consider in population-level association research studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.