ArticlePLoS biology2019
High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation-associated versus preneurulation-associated fate patterning.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Human stem cell-based embryo models: from self-organization to recapitulating development.Frontiers in cell and developmental biology · 2026Review
- Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.bioRxiv : the preprint server for biology · 2025Article
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells.Stem cell reports · 2025Article
- Recapitulation and investigation of human brain development with neural organoids.IBRO neuroscience reports · 2024Article
- Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids.Frontiers in bioengineering and biotechnology · 2024Article
- Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Virtual cells in a virtual microenvironment recapitulate early development-like patterns in human pluripotent stem cell colonies.Stem cell reports · 2023Article
- Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device.Organs-on-a-chip · 2022Article
- Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells.Molecular systems biology · 2022Article
- Regulation of Nodal signaling propagation by receptor interactions and positive feedback.eLife · 2022Article
- A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation.Acta biomaterialia · 2022Article
- Article
- Engineering multiscale structural orders for high-fidelity embryoids and organoids.Cell stem cell · 2022Review
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- Plating human iPSC lines on micropatterned substrates reveals role for ITGB1 nsSNV in endoderm formation.Stem cell reports · 2021Article
- Quantitative developmental biology in vitro using micropatterning.Development (Cambridge, England) · 2021Review
- Phenotypic technologies in stem cell biology.Cell chemical biology · 2021Review
Corrections and comments
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Authors and funding
17 authors.
Funding
Abstract
In vitro models of postimplantation human development are valuable to the fields of regenerative medicine and developmental biology. Here, we report characterization of a robust in vitro platform that enabled high-content screening of multiple human pluripotent stem cell (hPSC) lines for their ability to undergo peri-gastrulation-like fate patterning upon bone morphogenetic protein 4 (BMP4) treatment of geometrically confined colonies and observed significant heterogeneity in their differentiation propensities along a gastrulation associable and neuralization associable axis. This cell line-associated heterogeneity was found to be attributable to endogenous Nodal expression, with up-regulation of Nodal correlated with expression of a gastrulation-associated gene profile, and Nodal down-regulation correlated with a preneurulation-associated gene profile expression. We harness this knowledge to establish a platform of preneurulation-like fate patterning in geometrically confined hPSC colonies in which fates arise because of a BMPs signalling gradient conveying positional information. Our work identifies a Nodal signalling-dependent switch in peri-gastrulation versus preneurulation-associated fate patterning in hPSC cells, provides a technology to robustly assay hPSC differentiation outcomes, and suggests conserved mechanisms of organized fate specification in differentiating epiblast and ectodermal tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.