Evidence map›Paper›PMID 31633779›Full record

ArticleBioinformatics (Oxford, England)2020

3D flow field estimation and assessment for live cell fluorescence microscopy.

Sandeep Manandhar, Patrick Bouthemy, Erik Welf, Gaudenz Danuser, Philippe Roudot, Charles Kervrann

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sandeep ManandharInria, Centre Rennes-Bretagne Atlantique, Rennes 35042, France.
Patrick BouthemyInria, Centre Rennes-Bretagne Atlantique, Rennes 35042, France.
Erik WelfLyda Hill Department of Bioinformatics, UTSouthwestern, Dallas, TX 75390, USA.
Gaudenz DanuserLyda Hill Department of Bioinformatics, UTSouthwestern, Dallas, TX 75390, USA.
Philippe RoudotLyda Hill Department of Bioinformatics, UTSouthwestern, Dallas, TX 75390, USA.
Charles KervrannInria, Centre Rennes-Bretagne Atlantique, Rennes 35042, France.

Funding

Quantitative Fluorescent Speckle MicroscopyR01GM067230 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI DANUSER, GAUDENZ · 2003 to 2019
$3.3M
Mechanisms of melanoma migration and survival in 3D microenvironmentsK25CA204526 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI WELF, ERIK S · 2016 to 2020
$585k
NCI NIH HHS K25 CA204526NIGMS NIH HHS R01 GM067230
6 · The paper itself

Abstract

motivationThe revolution in light sheet microscopy enables the concurrent observation of thousands of dynamic processes, from single molecules to cellular organelles, with high spatiotemporal resolution. However, challenges in the interpretation of multidimensional data requires the fully automatic measurement of those motions to link local processes to cellular functions. This includes the design and the implementation of image processing pipelines able to deal with diverse motion types, and 3D visualization tools adapted to the human visual system.

resultsHere, we describe a new method for 3D motion estimation that addresses the aforementioned issues. We integrate 3D matching and variational approach to handle a diverse range of motion without any prior on the shape of moving objects. We compare different similarity measures to cope with intensity ambiguities and demonstrate the effectiveness of the Census signature for both stages. Additionally, we present two intuitive visualization approaches to adapt complex 3D measures into an interpretable 2D view, and a novel way to assess the quality of flow estimates in absence of ground truth. AVAILABILITY AND IMPLEMENTATION: https://team.inria.fr/serpico/data/3d-optical-flow-data/. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Indexed as

Image Processing, Computer-AssistedImaging, Three-DimensionalAlgorithmsHumansMicroscopy, FluorescenceMotion

Identifiers

PMID31633779
PMCPMC7523654

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.