Evidence map›Paper›PMID 31632535›Full record

ArticleAmerican journal of translational research2019

Long non-coding RNA FOXD3-AS1 aggravates ischemia/reperfusion injury of cardiomyocytes through promoting autophagy.

Guoxin Tong, Yongmei Wang, Chenke Xu, Yizhou Xu, Xianhua Ye, Liang Zhou, Gangjie Zhu, Zhanlin Zhou, Jinyu Huang

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.

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  17. Long Noncoding RNAs in Myocardial Ischemia-Reperfusion Injury.Oxidative medicine and cellular longevity · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Guoxin TongDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Yongmei WangDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Chenke XuDepartment of Ultrasound, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Yizhou XuDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Xianhua YeDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Liang ZhouDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Gangjie ZhuDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Zhanlin ZhouDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Jinyu HuangDepartment of Cardiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, People's Republic of China.
Hangzhou First People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We aimed to investigate the role of long non-coding RNA (lncRNA) FOXD3 antisense RNA 1 (FOXD3-AS1) in myocardial Ischemia/reperfusion (I/R) injury. In our study, H9C2 cells were treated with oxygen-glucose deprivation and reoxygenation (OGD/R). RT-qPCR was performed to detect the expression level of lncRNA FOXD3-AS1 in OGD/R induced H9C2 cells. Then, pcDNA-lncRNA FOXD3-AS1 was transfected into H9C2 cells. The level of LC3 II was measured by immunofluorescence assay. And the expression of autophagy related genes were detected using western blot. In addition, 3-methyladenine (3 M), an autophagy inhibitor, was recruited to treat with H9C2 cells. The contents of creatine kinase (CK), CK isoenzymes (CK-MB), cardiac troponin I (cTnI), inflammation associated factors, reactive oxygen (ROS) and NO were evaluated by kits. Moreover, cell apoptosis was measured by a flow cytometry assay and the expression levels of apoptosis associated proteins were evaluated by western blot. Furthermore, the expression of NF-κB/iNOS/COX2 signaling were measured in our study. The results indicated that FOXD3-AS1 expression was increased in OGD/R-treated H9C2 cells and overexpression of FOXD3-AS1 upregulated the expression of LC3 II, Beclin1, ATG5 accompanied by a downregulated expression of p62. In addition, FOXD3-AS1 overexpression increased the levels of CK, CK-MB, cTnI, TNF-α, IL-1β, IL-6, ROS and NO, whereas the increase of above factors were reversed following treatment with 3 M. Moreover, FOXD3-AS1 overexpression enhanced the rate of apoptosis cells coupled with a decrease of Bcl-2 expression and an increase of Bax and cleaved caspase 3 expression, which were reversed by 3 M. Furthermore, FOXD3-AS1 overexpression promoted the activation of NF-κB/iNOS/COX2 signaling, which was blocked following treatment with 3 M. These findings demonstrate that overexpression of lncRNA FOXD3-AS1 aggravates myocardial I/R injury through promoting autophagy, which was regulated by activating NF-κB/iNOS/COX2 signaling.

Indexed as

apoptosisautophagycardiomyocytesFOXD3-AS1ischemia/reperfusion

Identifiers

PMID31632535
PMCPMC6789269
OpenAlexW2985408467

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.