ArticleMethods in enzymology2019
Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase.
Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Substrate energetics in non-templated DNA synthesis by terminal deoxynucleotidyl transferase.Nucleic acids research · 2026Article
- Synthetic intrinsically disordered protein fusion tags that enhance protein solubility.Nature communications · 2024Article
- Multiplex enzymatic synthesis of DNA with single-base resolution.Science advances · 2023Article
- DNA synthesis technologies to close the gene writing gap.Nature reviews. Chemistry · 2023Review
- Insight into the mechanism of DNA synthesis by human terminal deoxynucleotidyltransferase.Life science alliance · 2022Article
- The Protein-Templated Synthesis of Enzyme-Generated Aptamers.Angewandte Chemie (International ed. in English) · 2022Article
- Programmable Site-Specific Functionalization of DNA Origami with Polynucleotide Brushes.Angewandte Chemie (International ed. in English) · 2021Article
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Authors and funding
4 authors.
Funding
Abstract
The recognition that nucleic acids can be used as polymeric materials led to the blossoming of the field of DNA nanotechnology, with a broad range of applications in biotechnology, biosensors, diagnostics, and drug delivery. These applications require efficient methods to synthesize and chemically modify high molecular weight DNA. Here, we discuss terminal deoxynucleotidyl transferase (TdT)-catalyzed enzymatic polymerization (TcEP) as an alternative to conventional enzymatic and solid-phase DNA synthesis. We describe biochemical requirements for TcEP and provide step-by-step protocols to carry out TcEP in solution and from surfaces.
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Registered trials
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