Evidence map›Paper›PMID 31629364›Full record

ArticlePediatric research2020

Generation of infant- and pediatric-derived urinary induced pluripotent stem cells competent to form kidney organoids.

Jaap Mulder, Sazia Sharmin, Theresa Chow, Deivid Carvalho Rodrigues, Matthew R Hildebrandt, Robert D'Cruz, Ian Rogers, James Ellis, Norman D Rosenblum

Abstract read
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In one paragraph

Article in Pediatric research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

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  12. Organs-on-chip technology: a tool to tackle genetic kidney diseases.Pediatric nephrology (Berlin, Germany) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jaap MulderDivision of Nephrology, Department of Pediatrics, The Hospital for Sick Children, Toronto, ON, Canada.
Sazia SharminProgram in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Theresa ChowLunenfeld-Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada.
Deivid Carvalho RodriguesProgram in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Matthew R HildebrandtProgram in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Robert D'CruzProgram in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Ian RogersLunenfeld-Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada.
James EllisProgram in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Norman D RosenblumDivision of Nephrology, Department of Pediatrics, The Hospital for Sick Children, Toronto, ON, Canada. norman.rosenblum@sickkids.ca.

Funding

CIHR
6 · The paper itself

Abstract

backgroundHuman induced pluripotent stem cells (iPSCs) are a promising tool to investigate pathogenic mechanisms underlying human genetic conditions, such as congenital anomalies of the kidney and urinary tract (CAKUT). Currently, iPSC-based research in pediatrics is limited by the invasiveness of cell collection.

methodsUrine cells (UCs) were isolated from pediatric urine specimens, including bag collections, and reprogrammed using episomal vectors into urinary iPSCs (UiPSCs). Following iPSC-quality assessment, human kidney organoids were generated.

resultsUCs were isolated from 71% (12/17) of single, remnant urine samples obtained in an outpatient setting (patients 1 month-17 years, volumes 10-75 ml). Three independent UCs were reprogrammed to UiPSCs with early episome loss, confirmed pluripotency and normal karyotyping. Subsequently, these UiPSCs were successfully differentiated into kidney organoids, closely resembling organoids generated from control fibroblast-derived iPSCs. Importantly, under research conditions with immediate sample processing, UC isolation was successful 100% for target pediatric CAKUT patients and controls (11/11) after at most two urine collections.

conclusionsUrine in small volumes or collected in bags is a reliable source for reprogrammable somatic cells that can be utilized to generate kidney organoids. This constitutes an attractive approach for patient-specific iPSC research involving infants and children with wide applicability and a low threshold for participation.

Indexed as

Cell SeparationAdolescentCase-Control StudiesCell ProliferationCells, CulturedCellular ReprogrammingCellular Reprogramming TechniquesChildChild, PreschoolFeasibility StudiesFemaleGene Expression Regulation, DevelopmentalHumansInduced Pluripotent Stem CellsInfantKidney

Identifiers

PMID31629364

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.