ArticleNature communications2019
DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Effects of Ligand Mobility on Ligand-Receptor Interactions Driving Integrin-Mediated Cell Adhesion.Chemical & biomedical imaging · 2026Article
- Super-Resolution Axial Imaging for Quantifying Piconewton Traction Forces in Live Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Engineered In Vitro Platforms for Mechanochemical Control of Cell Migration.Cold Spring Harbor perspectives in biology · 2025Review
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors.Nature nanotechnology · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Photonic control of ligand nanospacing in self-assembly regulates stem cell fate.Bioactive materials · 2024Article
- Molecular Compressive Force Sensor for Mapping Forces at the Cell-Substrate Interface.Journal of the American Chemical Society · 2024Article
- Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing.Nature methods · 2023Article
- Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand.Nature biomedical engineering · 2023Article
- Multiplexed Detection of Molecular Interactions with DNA Origami Engineered Cells in 3D Collagen Matrices.ACS applied materials & interfaces · 2022Article
- Excited State Charge-Transfer Complexes Enable Fluorescence Color Changes in a Supramolecular Cyclophane Mechanophore.Angewandte Chemie (International ed. in English) · 2022Article
- Mechanosensing view of SARS-CoV-2 infection by a DNA nano-assembly.Cell reports. Physical science · 2022Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- DNA Tension Probes Show that Cardiomyocyte Maturation Is Sensitive to the Piconewton Traction Forces Transmitted by Integrins.ACS nano · 2022Article
- Recent developments in DNA-based mechanical nanodevices.Chemical communications (Cambridge, England) · 2022Review
- The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation.Science advances · 2022Article
- Single-Molecule Force Imaging Reveals That Podosome Formation Requires No Extracellular Integrin-Ligand Tensions or Interactions.ACS nano · 2022Article
- Matrix Metalloproteinases Shape the Tumor Microenvironment in Cancer Progression.International journal of molecular sciences · 2021Review
- Obtaining Precise Molecular Information via DNA Nanotechnology.Membranes · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Podosomes are ubiquitous cellular structures important to diverse processes including cell invasion, migration, bone resorption, and immune surveillance. Structurally, podosomes consist of a protrusive actin core surrounded by adhesion proteins. Although podosome protrusion forces have been quantified, the magnitude, spatial distribution, and orientation of the opposing tensile forces remain poorly characterized. Here we use DNA nanotechnology to create probes that measure and manipulate podosome tensile forces with molecular piconewton (pN) resolution. Specifically, Molecular Tension-Fluorescence Lifetime Imaging Microscopy (MT-FLIM) produces maps of the cellular adhesive landscape, revealing ring-like tensile forces surrounding podosome cores. Photocleavable adhesion ligands, breakable DNA force probes, and pharmacological inhibition demonstrate local mechanical coupling between integrin tension and actin protrusion. Thus, podosomes use pN integrin forces to sense and respond to substrate mechanics. This work deepens our understanding of podosome mechanotransduction and contributes tools that are widely applicable for studying receptor mechanics at dynamic interfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.