ArticleNature communications2019
Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 108 citations in OpenAlex.
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Diversification of the 10th core atom of 9-cyanopyronins expands the resonance Raman vibrational palette.Communications chemistry · 2026Article
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Fluorescence-free single-molecule microscopy via electronic resonance stimulated Raman scattering.Nature communications · 2026Article
- Electric fields at hydrophobic water interfaces: spectroscopic evidence, physical origin, and implications on reactivity.Chemical Society reviews · 2026Review
- Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025Article
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Article
- Single-Entity Resolution Single-Cell Nanosensor Reveals Reactive Oxygen Species at Stress Granules Are Formed by Interfacial Redox Chemistry.Journal of the American Chemical Society · 2025Article
- Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels.Nature biomedical engineering · 2025Article
- Condenzymes: Biomolecular condensates with inherent catalytic activities.bioRxiv : the preprint server for biology · 2025Article
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate.Optica · 2025Article
- An exchangeable SIM probe for monitoring organellar dynamics of necrosis cells and intracellular water heterogeneity in kidney repair.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Room-Temperature Single-Molecule Infrared Imaging and Spectroscopy through Bond-Selective Fluorescence.Angewandte Chemie (International ed. in English) · 2024Article
- Raman spectroscopy in the study of amyloid formation and phase separation.Biochemical Society transactions · 2024Review
- Nitrile Vibrational Lifetimes as Probes of Local Electric Fields.The journal of physical chemistry letters · 2024Article
- Water bend-libration as a cellular Raman imaging probe of hydration.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Activatable Raman Probes Utilizing Enzyme-Induced Aggregate Formation for SelectiveJournal of the American Chemical Society · 2023Article
- Bringing Vibrational Imaging to Chemical Biology with Molecular Probes.ACS chemical biology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack of proper water-sensing tools with high sensitivity and spatial resolution. Recently, stimulated Raman excited fluorescence (SREF) microscopy was reported as the most sensitive vibrational imaging in the optical far field. Herein we develop SREF into a water-sensing tool by coupling it with vibrational solvatochromism. This technique allows us to directly visualize spatially-resolved distribution of water states inside single mammalian cells. Qualitatively, our result supports the concept of biological water and reveals intracellular water heterogeneity between nucleus and cytoplasm. Quantitatively, we unveil a compositional map of the water pool inside living cells. Hence we hope SREF will be a promising tool to study intracellular water and its relationship with cellular activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.